Nanog antibody (mAb) (Clone 2E6E1)

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

ICC, IF, WB

CloneNo.

2E6E1

Cat No : 61627,61628

Synonyms

Nanog, Nanog Homeobox, Homeobox Transcription Factor Nanog, hNanog, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48, antibody, monoclonal, mAb, wb, western blotting, sample, immunofluorescence, immunocytochemistry, IF, ICC, transcription regulator, stem cells, embryonic stem cells, es, smad1, activator, repressor, sacrococcygeal teratoma, ovarian germ cell tumor

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Validation Data Gallery

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  Nanog antibody (mAb) (Clone 2E6E1) tested by immunofluorescence. Top: HeLa cells stained with Nanog antibody (mAb). Bottom: Hoechst staining.

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  Nanog antibody (mAb) (Clone 2E6E1) tested by Western blot. Nanog antibody detection by Western blot. The analysis was performed using 30 ug of mouse ES nuclear extract and Nanog antibody at a 2 ug/ml dilution.

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Product Information

• Tested Applications: ICC, IF, WB

  Applications Validated by Active Motif: ICC/IF: 2 - 10 ug/ml dilution WB*: 1 - 2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91251.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: This antibody was raised against a peptide comprising amino acids 2-25 of mouse Nanog.
• Full Name: Nanog antibody (mAb) (Clone 2E6E1)
• Synonyms: Nanog, Nanog Homeobox, Homeobox Transcription Factor Nanog, hNanog, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48, antibody, monoclonal, mAb, wb, western blotting, sample, immunofluorescence, immunocytochemistry, IF, ICC, transcription regulator, stem cells, embryonic stem cells, es, smad1, activator, repressor, sacrococcygeal teratoma, ovarian germ cell tumor
• Molecular weight: 45 kDa
• GenBank accession number: NP_001276757
• RRID: AB_2793709
• Purification Method: Protein G Chromatography
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Nanog (Nanog homeobox) is a transcriptional regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation (By similarity).