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NCAPH2 antibody (mAb) (Clone 5F2G4)

Host / Isotype

Rat / IgG2a

Reactivity

Human

Applications

ICC, IF, WB

CloneNo.

5F2G4

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Cat No : 61681,61682 61681

Synonyms

NCAPH2, Non-SMC Condensin II Complex, Subunit H2, CTA-384D8.36, CAPH2, Condensin-2 Complex Subunit H2, CAP-H2 Subunit Of The Condensin II Complex, Kleisin-beta, Kleisin Beta, hCAP-H2, Chromosome-Associated Protein H2, Non-SMC Condensin II Complex Subunit H2, clone 5F2G4, immunocytochemistry, icc, immunofluorescence, if, western blotting, wb, antibody, antibodies, monoclonal, mab, sample

Validation Data Gallery

  • NCAPH2 antibody (mAb) (Clone 5F2G4) tested by immunofluorescence. Formaldehyde fixed HeLa cell stained with the NCAPH2 antibody.

    NCAPH2 antibody (mAb) (Clone 5F2G4) tested by immunofluorescence. Formaldehyde fixed HeLa cell stained with the NCAPH2 antibody.

  • NCAPH2 antibody (mAb) (Clone 5F2G4) tested by Western blot. NCAPH2 antibody detection by Western blot. The analysis was performed using 30 ug of HeLa nuclear extract and NCAPH2 antibody at a 1 ug/ml dilution.

    NCAPH2 antibody (mAb) (Clone 5F2G4) tested by Western blot. NCAPH2 antibody detection by Western blot. The analysis was performed using 30 ug of HeLa nuclear extract and NCAPH2 antibody at a 1 ug/ml dilution.

Product Information

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 1 - 2 ug/ml dilution WB*: 0.5 - 2 ug/ml dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against a recombinant protein to human NCAPH2.
Full Name NCAPH2 antibody (mAb) (Clone 5F2G4)
Synonyms NCAPH2, Non-SMC Condensin II Complex, Subunit H2, CTA-384D8.36, CAPH2, Condensin-2 Complex Subunit H2, CAP-H2 Subunit Of The Condensin II Complex, Kleisin-beta, Kleisin Beta, hCAP-H2, Chromosome-Associated Protein H2, Non-SMC Condensin II Complex Subunit H2, clone 5F2G4, immunocytochemistry, icc, immunofluorescence, if, western blotting, wb, antibody, antibodies, monoclonal, mab, sample
Molecular weight 90 kDa
GenBank accession numberNP_001171940
RRIDAB_2793733
Purification Method Protein G Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

NCAPH2 (Non-SMC Condensin II Complex, Subunit H2) Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development. (By similarity)