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N-Myc antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP-Seq, WB

Activ Motif logo

Cat No : 61185,61186 61185

Synonyms

N-Myc, MYCN, Myc family, proto-oncogene, oncogene, proliferation, nervous system, neural, neuroblastoma, medulloblastoma, retinoblastoma, dbdabs, cancer, MYC, sample

Validation Data Gallery

  • N-Myc antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 200 ug of chromatin from human cell line chromatin and 20 ug N-Myc antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9.2 million sequence tags were mapped to identify N-Myc binding sites on chromosome 3. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

    N-Myc antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 200 ug of chromatin from human cell line chromatin and 20 ug N-Myc antibody. ChIP DNA was sequenced on the Illumina HiSeq and 9.2 million sequence tags were mapped to identify N-Myc binding sites on chromosome 3. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

  • N-Myc antibody (pAb) tested by Western blot. Nuclear extract (20 ug) of Kelly cells probed with N-Myc antibody at a dilution of 1:1,000

    N-Myc antibody (pAb) tested by Western blot. Nuclear extract (20 ug) of Kelly cells probed with N-Myc antibody at a dilution of 1:1,000

Product Information

Tested Applications ChIP-Seq, WB

Applications Validated by Active Motif: ChIP-Seq: 10 - 20 ul per ChIP WB: 1:500 - 1:2,000 dilution For optimal results in Western Blot, primary antibody incubations should be performed at 4°C. Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This N-Myc antibody was raised against a peptide derived from the C-terminal region of human N-Myc.
Full Name N-Myc antibody (pAb)
Synonyms N-Myc, MYCN, Myc family, proto-oncogene, oncogene, proliferation, nervous system, neural, neuroblastoma, medulloblastoma, retinoblastoma, dbdabs, cancer, MYC, sample
Molecular weight 60 kDa
GenBank accession numberNP_005369
RRIDAB_2793543
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

N-Myc (MYCN) is a member of the Myc family of proto-oncogenes. Myc family members play crucial roles in regulating cell proliferation, size and differentiation. N-Myc is required for proper development of the nervous system. Myc family proteins can induce malignant transformation inappropriately modulating gene transcription, leading to unchecked cell proliferation. Misexpression of N-Myc is often associated with pediatric neural cancers such as neuroblastoma, medulloblastoma and retinoblastoma.