Mouse Control qPCR Primer Sets

Synonyms

chip, chromatin IP, chromatin immunoprecipitation, controls, PCR primers, primer sets, qPCR, endpoint PCR, end-point, mouse, positive control, Zc3h13 exon 10, 71022

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Overview

Mouse Control qPCR Primer Sets were designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with mouse samples. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR and endpoint PCR of ChIP samples from multiple mouse cell lines.

Click the Mouse qPCR Primer Sets tab for information on the target of each primer set and what it is an appropriate control for.

Mouse qPCR Primer Sets

Mouse Chromatin IP Control qPCR Primer Sets

Cat. No.	Name	Gene	Control for...
71011	Mouse Negative Control Primer Set 1	Gene desert on chromosome 6	Any transcription factor, LSD1, RNA pol II, 5-mC (MeDIP), H3K9ac, H3K14ac, H3K27ac, H3K4me1, H3K4me2, H3K4me3, H3K36me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac
71012	Mouse Negative Control Primer Set 2	Gene desert on chromosome 17	Any transcription factor, LSD1, RNA pol II, 5-mC (MeDIP), H3K9ac, H3K14ac, H3K27ac, H3K4me1, H3K4me2, H3K4me3, H3K36me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac
71013	Mouse Negative Control Primer Set 3	Actb promoter	H3K27me3, EZH2
71014	Mouse Negative Control Primer Set 4	Gapdh promoter	H3K27me3, EZH2
71015	Mouse Positive Control Primer Set Actb-1	Actb intron 3	RNA pol II phospho Ser2, H3K36me3
71016	Mouse Positive Control Primer Set Gapdh-1	Gapdh intron 2	RNA pol II phospho Ser2, H3K36me3
71017	Mouse Positive Control Primer Set Actb-2	Actb promoter	H3K9ac, H3K14ac, H3K4me2, H3K4me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac, HDAC1, LSD1, Total RNA pol II, RNA pol II phospho Ser5
71018	Mouse Positive Control Primer Set Gapdh-2	Gapdh promoter	H3K9ac, H3K14ac, H3K4me2, H3K4me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac, Total RNA pol II, RNA pol II phospho Ser5
71019	Mouse Positive Control Primer Set Hoxc10	Hoxc10 intron 1	H3K27me3, EZH2
71020	Mouse Positive Control Primer Set Pax-2	Region 5´ to Pax-2	H3K27me3,EZH2
71021	Mouse Positive Control Primer Set Grik3	Grik3 intron 8	5-mC (MeDIP)
71022	Mouse Positive Control Primer Set Zc3h13	Zc3h13 exon 10	5-mC (MeDIP)

Contents

Contents & Storage

Each control primer set contains both forward and reverse primers in 400 µl of nuclease-free distilled water. The final concentration for each primer is 2.5 µM.

The primer sets are shipped at room temperature and should be stored at -20°C upon receipt. The control primer sets are guaranteed stable for 6 months from date of receipt when stored properly.

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