Model Organism Control qPCR Primer Sets

Synonyms

chip, chromatin IP, chromatin immunoprecipitation, controls, PCR primers, primer sets, qPCR, endpoint PCR, end-point, zebrafish, danio rerio, positive control, bactin1, 71036

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Overview

Model Organism Control qPCR Primer Sets were designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with samples from rat, zebrafish, Drosophila or budding yeast. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR and endpoint PCR of ChIP DNA from multiple samples.

Click the Model Organism qPCR Primer Sets tab for information on the target of each primer set and what it is an appropriate control for.

Model Organism qPCR Primer Sets

Model Organism Chromatin IP Control qPCR Primer Sets

Cat. No.	Name	Gene	Control for...
71024	Rat Negative Control Primer Set 1	Gene desert on rat chromosome 17	Any transcription factor, RNA pol II and most histone modifications
71025	Rat Negative Control Primer Set 2	Gene desert on rat chromosome 5	Any transcription factor, RNA pol II and most histone modifications
71026	Rat Positive Control Primer Set Actb	Exon 6 of rat Actb gene	RNA pol II phospho Ser2
71027	Rat Positive Control Primer Set Gapdh	Intron 1 of rat Gapdh gene	RNA pol II phospho Ser2
71028	Drosophila Negative Control Primer Set 1	Gene desert on Drosophila chromosome 3L	Any transcription factor, RNA pol II and most histone modifications
71029	Drosophila Negative Control Primer Set 2	Gene desert on Drosophila chromosome 2R	Any transcription factor, RNA pol II and most histone modifications
71030	Drosophila Positive Control Primer Set Act5C	Intron 1 of Act5C gene	RNA pol II phospho Ser2
71031	Drosophila Positive Control Primer Set Gapdh1	Gapdh promoter	H3K4me2, H3K4me3
71032	S. cerevisiae Negative Control Primer Set 1	Intergenic region on chromosome 5	Any transcription factor, RNA pol II and most histone modifications
71033	S. cerevisiae Positive Control Primer Set ACT1	Coding region of ACT1 gene	RNA pol II phospho Ser2
71034	Zebrafish Negative Control Primer Set 1	Gene desert on chromosome 3	Any transcription factor, RNA pol II and most histone modifications
71035	Zebrafish Negative Control Primer Set 2	Gene desert on chromosome 7	Any transcription factor, RNA pol II and most histone modifications
71036	Zebrafish Positive Control Primer Set bactin	Intron 1 of bactin1 gene	H3K4me3

Contents

Contents & Storage

Each control primer set contains both forward and reverse primers in 400 µl of nuclease-free distilled water. The final concentration for each primer is 2.5 µM.

The primer sets are shipped at room temperature and should be stored at -20°C upon receipt. The control primer sets are guaranteed stable for 6 months from date of receipt when stored properly.

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