Methylated DNA Standard Kit

Synonyms

DNA methylation, control DNA, human DNA, DNA, 5-Hydroxymethylcytidine, 5-hydroxymethylcytosine, 5-hmC, 5-Hydroxy methylcytidine, 5-hydroxy methyl cytidine, 5-hydroxymethylcytosine, 5-hmc, 5 hmc, 5hmc, hydroxy methyl cytosine, Hydroxymethylcytosine, Hydroxy methylcytosine, Hydroxy methyl cytosine, 5-methylcytosine, 5hmC, 5- methylcytidine, 5- methyl cytidine, 5-methylcytosine, 5-mc, 5 mc, 5mc, methyl cytosine, methylcytosine, methylcytosine, methyl cytosine

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Overview

Active Motif's Methylated DNA Standard Kit includes three recombinant double-stranded DNA standards derived from the APC gene promoter: unmethylated DNA, 5-methylcytosine methylated DNA and 5-hydroxymethylcytosine methylated DNA. The DNA standards can be used as positive controls in methylation analysis experiments, or used in combination with Active Motif's antibodies to the different types of DNA methylation. The DNA standards are suitable for use in dot blot, or spiked recovery immunoprecipitation assays. The Methylated DNA Standard Kit also includes positive control PCR primers specific to the APC promoter which can be used for real time or endpoint PCR analysis.

Data

Figure 1: Agarose gel analysis of Methylated DNA Standard Kit samples.
500 ng of each DNA standard was loaded on a 2.5% agarose gel. Lane 1: unmethylated DNA. Lane 2: 5-methylcytosine containing DNA. Lane 3: 5-hydroxymethylcytosine containing DNA.

Figure 2: Dot blot analysis Methylated DNA Standard Kit samples.
DNA (50 ng) from the three different samples was spotted onto a positively charged nylon membrane. Top panel: Dot blot probed with 5-Methylcytidine antibody (Clone 33D3) (Catalog No. 39649, 1:1,000 dilution). Bottom panel: Dot blot probed with 5-Hydroxymethylcytidine antibody (Catalog No. 39769, 1:5,000 dilution). Lane 1: Single stranded DNA. Lane 2: Double stranded DNA.

Contents

Contents & Storage

This kit contains 3 x 2.5 µg each DNA amplified from the APC gene promoter (50 ng/µl) and APC Primer mix (3 x 400 µl each, 2.5 µM concentration). The APC PCR Primer mix produces a 195 bp amplicon.

Store at -20°C. This product is guaranteed stable for 6 months from date of receipt when stored properly.

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