MeCP2 antibody (mAb) (Clone 4H7)
Host / Isotype
Rat / IgG2a
Reactivity
Human, Mouse, Rat
Applications
ChIP, ICC, IF, IHC, IP, WB
CloneNo.
4H7
Cat No : 61291,61292 61291
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
ChIP, ICC, IF, IHC, IP, WB
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications: ChIP IP ICC/IF IHC WB View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody. |
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This MeCP2 antibody was raised against a full-length protein corresponding to rat MeCP2. |
| Full Name | MeCP2 antibody (mAb) (Clone 4H7) |
| Synonyms | MeCP2, MeCp-2 protein, RS, RTS, RTT, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13, antibody, mAb, sample, monoclonal |
| Molecular weight | 72 kDa |
| GenBank accession number | NP_073164 |
| RRID | AB_2572208 |
| Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl CpG binding protein 2, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A.