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MED1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

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Cat No : 61065,61066 61065

Synonyms

MED1, Mediator complex subunit 1, TRIP2, Thyroid receptor-interacting protein 2, Mediator complex, RNA polymerase II, hematopoietic stem cell, hsc, hematopoietic progenitor cell function, stem cell, stem cells, polyclonal, pab, antibody, antibodies, western blotting, wb, immunoprecipitation, ip, sample

Validation Data Gallery

  • MED1 antibody (pAb) tested by Immunoprecipitation. 10 ul of MED1 antibody was used to immunoprecipitate MED1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the MED1 antibody at a dilution of 1:2,000.

    MED1 antibody (pAb) tested by Immunoprecipitation. 10 ul of MED1 antibody was used to immunoprecipitate MED1 from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the MED1 antibody at a dilution of 1:2,000.

  • MED1 antibody (pAb) tested by Western blot. Nuclear extract (30 ug) of HeLa cells probed with MED1 antibody at a dilution of 1:2,000.

    MED1 antibody (pAb) tested by Western blot. Nuclear extract (30 ug) of HeLa cells probed with MED1 antibody at a dilution of 1:2,000.

Product Information

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This MED1 antibody was raised against a peptide within the C-terminal region of human MED1.
Full Name MED1 antibody (pAb)
Synonyms MED1, Mediator complex subunit 1, TRIP2, Thyroid receptor-interacting protein 2, Mediator complex, RNA polymerase II, hematopoietic stem cell, hsc, hematopoietic progenitor cell function, stem cell, stem cells, polyclonal, pab, antibody, antibodies, western blotting, wb, immunoprecipitation, ip, sample
Molecular weight 210 kDa
GenBank accession numberNP_004765
RRIDAB_2714185
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

MED1 (Mediator complex subunit 1, TRIP2, Thyroid receptor-interacting protein 2) is a component of the Mediator complex, a coactivator involved in the regulated transcription of most genes transcribed by RNA polymerase II. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. MED1 is involved in supporting hematopoietic stem and/or progenitor cell function.