MCM2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 61717

Synonyms

MCM2, Minichromosome Maintenance Complex Component 2, CDCL1, Minichromosome Maintenance Protein 2 Homolog, Nuclear Protein BM28, CCNL1, BM28, MCM2 Minichromosome Maintenance Deficient 2, Mitotin (S. Cerevisiae), Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin), MCM2 Minichromosome Maintenance Deficient 2, Mitotin, Minichromosome Maintenance Deficient 2 (Mitotin), DNA Replication Licensing Factor MCM2, Cell Devision Cycle-Like 1, Cyclin-Like 1, EC 3.6.4.12, KIAA0030, Mitotin, D3S3194, MITOTIN, Cdc19, antibody, antibodies, polyclonal, pab, wb, western blotting

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Validation Data Gallery

• 

  MCM2 antibody (pAb) tested by Western blot. MCM2 detection by Western blot. The analysis was performed using MCF7 nuclear cell extract (20 ug) and MCM2 antibody at a 1:1,000 dilution.

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB*: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This antibody was raised against a full-length recombinant protein corresponding to human MCM2.
• Full Name: MCM2 antibody (pAb)
• Synonyms: MCM2, Minichromosome Maintenance Complex Component 2, CDCL1, Minichromosome Maintenance Protein 2 Homolog, Nuclear Protein BM28, CCNL1, BM28, MCM2 Minichromosome Maintenance Deficient 2, Mitotin (S. Cerevisiae), Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin), MCM2 Minichromosome Maintenance Deficient 2, Mitotin, Minichromosome Maintenance Deficient 2 (Mitotin), DNA Replication Licensing Factor MCM2, Cell Devision Cycle-Like 1, Cyclin-Like 1, EC 3.6.4.12, KIAA0030, Mitotin, D3S3194, MITOTIN, Cdc19, antibody, antibodies, polyclonal, pab, wb, western blotting
• Molecular weight: 115 kDa
• GenBank accession number: NP_004517
• RRID: AB_2636824
• Purification Method: Affinity Purified
• Buffer: Purified IgG in PBS with 50% glycerol and 0.02% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

MCM2 (Minichromosome Maintenance Complex Component 2) acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.