Ki-67 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 39799,39800

Synonyms

Ki-67, Ki 67, Ki67, dividing cells, cancer, breast cancer, prostate cancer, antibody, antibodies, polyhclonal, sample

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Validation Data Gallery

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  Ki-67 antibody tested by Western blot. Detection of Ki-67 by Western blot. Raji nuclear extract (30 μg) probed with Ki-67 antibody (1:500 dilution). Lane 2: pre-incubation of antibody with immunizing peptide.

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB*: 1:500 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This Ki-67 antibody was raised against a peptide within the N-terminal region of human Ki-67.
• Full Name: Ki-67 antibody (pAb)
• Synonyms: Ki-67, Ki 67, Ki67, dividing cells, cancer, breast cancer, prostate cancer, antibody, antibodies, polyhclonal, sample
• Molecular weight: 360 kDa
• GenBank accession number: NP_002408
• RRID: AB_2793347
• Purification Method: Affinity Purified
• Buffer: Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Ki-67 encodes a protein that is highly expressed in dividing cells but not in quiescent cells. Ki-67 is a useful marker for rapidly dividing cells and has been used as a marker for cancer cells, Including breast, bladder, brain, colon, and prostate cancer.