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KLF6 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB

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Cat No : 61243,61244 61243

Synonyms

KLF6, Kruppel-like factor 6, B-cell-derived protein 1, BCD1, GC-rich sites-binding factor GBF, COPEB, ZF9, CPBP, Zf91, ST12, CBA1, GBF, GC-rich binding factor, PAC1, Krueppel-like factor 6, core promoter element binding protein, Kruppel-like zinc finger protein Zf9, Core promoter element-binding protein, protooncogene B-cell derived 1, Proto-oncogene BCD1, suppression of tumorigenicity 12 (prostate), Suppressor of tumorigenicity 12 protein, Transcription factor Zf9, antibody, pAb, polyclonal, sample, western blotting

Validation Data Gallery

  • KLF6 pAb tested by Western blot. 3T3-L1 nuclear extract extract following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) at 30 ug per lane was probed with KLF6 pAb at a dilution of 2 ug/ml . Lane 1: Day 0. Lane 2: Day 10 - fully differentiated adipocytes.

    KLF6 pAb tested by Western blot. 3T3-L1 nuclear extract extract following stimulation with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) at 30 ug per lane was probed with KLF6 pAb at a dilution of 2 ug/ml . Lane 1: Day 0. Lane 2: Day 10 - fully differentiated adipocytes.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB*: 0.5 - 2 ug/ml dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the internal region of human KLF6.
Full Name KLF6 antibody (pAb)
Synonyms KLF6, Kruppel-like factor 6, B-cell-derived protein 1, BCD1, GC-rich sites-binding factor GBF, COPEB, ZF9, CPBP, Zf91, ST12, CBA1, GBF, GC-rich binding factor, PAC1, Krueppel-like factor 6, core promoter element binding protein, Kruppel-like zinc finger protein Zf9, Core promoter element-binding protein, protooncogene B-cell derived 1, Proto-oncogene BCD1, suppression of tumorigenicity 12 (prostate), Suppressor of tumorigenicity 12 protein, Transcription factor Zf9, antibody, pAb, polyclonal, sample, western blotting
Molecular weight 40 kDa
GenBank accession numberNP_001291
RRIDAB_2793565
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

KLF6 (Kruppel-like factor 6) is member of the Kruppel-like family of transcription factors and is a zinc finger protein that acts as a transcriptional activator and functions as a tumor suppressor. Could play a role in B-cell growth and development. Multiple transcript variants encoding different isoforms have been found for this gene, some of which are implicated in carcinogenesis.