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JunD antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP-Seq, IP, WB

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Cat No : 61403,61404 61403

Synonyms

JunD, jun D proto-oncogene, AP-1, activator protein 1, JunD-FL isoform, transcription factor jun-D, Jun D, Jun-D, AP1, AP 1, transcription factor, antibody, antibodies, polyclonal, pAb, western blotting, wb, immunoprecipitation, ip, sample

Validation Data Gallery

  • JunD antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human endometrial stromal cell chromatin and 4 ug of JunD antibody. ChIP DNA was sequenced on the Illumina HiSeq and 8.6 million sequence tags were mapped to identify JunD binding sites on chromosome 16. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

    JunD antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human endometrial stromal cell chromatin and 4 ug of JunD antibody. ChIP DNA was sequenced on the Illumina HiSeq and 8.6 million sequence tags were mapped to identify JunD binding sites on chromosome 16. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

  • JunD antibody (pAb) tested by Immunoprecipitation. 10 ul of JunD antibody was used to immunoprecipitate JunD from 250 ug of K562 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the JunD antibody at a dilution of 1:1,000.

    JunD antibody (pAb) tested by Immunoprecipitation. 10 ul of JunD antibody was used to immunoprecipitate JunD from 250 ug of K562 nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the JunD antibody at a dilution of 1:1,000.

  • JunD antibody (pAb) tested by Western blot. K-562 nuclear extract (20 ug) probed with JunD antibody at a dilution of 1:1,000.

    JunD antibody (pAb) tested by Western blot. K-562 nuclear extract (20 ug) probed with JunD antibody at a dilution of 1:1,000.

Product Information

Tested Applications ChIP-Seq, IP, WB

Applications Validated by Active Motif: ChIP-Seq: 2 - 5 ul per ChIP IP: 10 ul per IP WB: 1:500 - 1:2,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human JunD.
Full Name JunD antibody (pAb)
Synonyms JunD, jun D proto-oncogene, AP-1, activator protein 1, JunD-FL isoform, transcription factor jun-D, Jun D, Jun-D, AP1, AP 1, transcription factor, antibody, antibodies, polyclonal, pAb, western blotting, wb, immunoprecipitation, ip, sample
Molecular weight 40 & 45 kDa
GenBank accession numberNP_002220
RRIDAB_2793621
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

JunD (Jun D proto-oncogene) is a member of the JUN family and forms a component of the AP-1 transcription factor complex. JunD binds DNA as a dimer. Other AP-1 family members consist of c-Fos, Fra-1, Fra-2, FosB, c-Jun, and JunD.