IKKb antibody (mAb) (Clone 10AG2)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB
CloneNo.
10AG2
Cat No : 40906 40906
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 1 - 2 ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. |
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This IKKb antibody was raised against a 6xHis-Tag full-length protein corresponding to human IKKb. |
| Full Name | IKKb antibody (mAb) (Clone 10AG2) |
| Synonyms | IKKb, IKK-b, IKK b, IK Kb, IK K-b, IKKbeta, IKK-beta, IKK beta, Clone 10AG2, Clone, 10AG2, mAb, monoclonal, antibody, antibodies, nfkb |
| Molecular weight | 87 kDa |
| GenBank accession number | NP_001177649 |
| RRID | AB_2793429 |
| Purification Method | Affinity Purified |
| Buffer | PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
IKKb (IkB Kinase beta subunit) – The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB (NFkB p50 & NFkB p65). The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IkBa at Ser32 and Ser36. This results ultimately in the degradation of IkB and the activation and nuclear translocation of NFkB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKa is not required for activation of the IKK complex and the subsequent degradation of IkBa. Once activated, IKKb autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.