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IKKb antibody (mAb) (Clone 10A9B6)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IP, WB

CloneNo.

10A9B6

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Cat No : 40907 40907

Synonyms

IKKb, IKK-b, IKK b, IK Kb, IK K-b, IKKbeta, IKK-beta, IKK beta, Clone 10A9B6, 10A9B6, mAb, monoclonal, antibody, antibodies, nfkb

Validation Data Gallery

  • IKKb mAb (Clone 10A9B6) tested by Western blot. Immunoprecipitation of IKKb. 2 ug of IKKb mAb (Clone 10A9B6) was used to immunoprecipitate IKKb from 1 x 106 cells. The immunoprecipitated protein was detected by Western blotting using the same antibody. The IKKb protein detected was ~87 kDa in size.

    IKKb mAb (Clone 10A9B6) tested by Western blot. Immunoprecipitation of IKKb. 2 ug of IKKb mAb (Clone 10A9B6) was used to immunoprecipitate IKKb from 1 x 106 cells. The immunoprecipitated protein was detected by Western blotting using the same antibody. The IKKb protein detected was ~87 kDa in size.

Product Information

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 2 ug per IP WB: 1 - 2 ug/ml dilution Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen This IKKb antibody was raised against a 6xHis-Tag full-length protein corresponding to human IKKb.
Full Name IKKb antibody (mAb) (Clone 10A9B6)
Synonyms IKKb, IKK-b, IKK b, IK Kb, IK K-b, IKKbeta, IKK-beta, IKK beta, Clone 10A9B6, 10A9B6, mAb, monoclonal, antibody, antibodies, nfkb
Molecular weight 87 kDa
GenBank accession numberNP_001177649
RRIDAB_2793430
Purification Method Affinity Purified
Buffer PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

IKKb (IkB Kinase beta subunit) – The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB (NFkB p50 & NFkB p65). The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. Upon its activation, the IKK complex phosphorylates IkBa at Ser32 and Ser36. This results ultimately in the degradation of IkB and the activation and nuclear translocation of NFkB. While both IKK-alpha and IKK-beta subunits are Ser/Thr kinases, IKK beta appears to be the principal kinase, as IKKa is not required for activation of the IKK complex and the subsequent degradation of IkBa. Once activated, IKKb autophosphorylates, which decreases IKK activity, thereby preventing a prolonged activation of inflammation.