Human Control qPCR Primer Sets

Synonyms

chip, chromatin IP, chromatin immunoprecipitation, controls, PCR primers, primer sets, qPCR, endpoint PCR, end-point, human, negative control, ACT-B promoter, 71023

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Overview

Human Control qPCR Primer Sets were designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with human samples. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR and endpoint PCR of ChIP samples from multiple human cell lines.

Click the Human qPCR Primer Sets tab for information on the target of each primer set and what it is an appropriate control for.

Human qPCR Primer Sets

Human Chromatin IP Control qPCR Primer Sets

Cat. No.	Name	Gene	Control for...
71001	Human Negative Control Primer Set 1	Gene desert on chromosome 12	Any transcription factor, LSD1, RNA pol II, 5-mC (MeDIP), H3K9ac, H3K14ac, H3K27ac, H3K4me1, H3K4me2, H3K4me3, H3K36me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac
71002	Human Negative Control Primer Set 2	Gene desert on chromosome 4	Any transcription factor, RNA pol II, 5-mC (MeDIP), H3K9ac, H3K14ac, H3K27ac, H3K4me1, H3K4me2, H3K4me3, H3K36me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac
71023	Human Negative Control Primer Set 3	ACTB promoter	H3K27me3, EZH2
71003	Human Positive Control Primer Set ACTB-1	ACTB intron 3	RNA pol II phospho Ser2, H3K36me3
71004	Human Positive Control Primer Set GAPDH-1	GAPDH intron 7	RNA pol II phospho Ser2, H3K36me3
71005	Human Positive Control Primer Set ACTB-2	ACTB promoter	H3K9ac, H3K14ac, H3K4me2, H3K4me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac, H3K27ac, HDAC1, LSD1, Total RNA pol II, RNA pol II phospho Ser5
71006	Human Positive Control Primer Set GAPDH-2	GAPDH intron 1	H3K9ac, H3K14ac, H3K4me2, H3K4me3, H4K5ac, H4K8ac, H4K12ac, H4K16ac, H3K27ac, Total RNA pol II, RNA pol II phospho Ser5
71007	Human Positive Control Primer Set MYT1	MYT1 promoter	H3K27me3, EZH2
71008	Human Positive Control Primer Set CCND2	CCND2 promoter	H3K27me3, EZH2, Suz12
71009	Human Positive Control Primer Set ZC3H13	ZC3H13 exon 8	5-mC (MeDIP)
71010	Human Positive Control Primer Set GEMIN4	Region 3´ of GEMIN4	5-mC (MeDIP)

Contents

Contents & Storage

Each control primer set contains both forward and reverse primers in 400 µl of nuclease-free distilled water. The final concentration for each primer is 2.5 µM.

The primer sets are shipped at room temperature and should be stored at -20°C upon receipt. The control primer sets are guaranteed stable for 6 months from date of receipt when stored properly.

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