Histone H4K12ac antibody (mAb) (Clone MABI 0412)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Wide Range Predicted

Applications

ChIP, DB, WB

CloneNo.

MABI 0412

Cat No : 61527

Synonyms

histone H4, histone, H4, histone-H4, histoneH4, mAb, monoclonal, mabi, H4 AcK12, Histone H4 Acetyl Lys12, Histone H4 Acetyl K12, Histone H4 Ac Lys12, H4 Acetyl Lys12, H4 Acetyl K12, H4 Ac Lys12, Histone H4 Acetylated Lysine 12, Histone H4 Acetyl Lysine12, H4 Acetylated Lysine 12, H4 Acetyl Lysine12, antibody, antibodies, H4K12Ac, chromatin, nucleosome, chromatin immunoprecipitation, chip, western blotting, wb

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Validation Data Gallery

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  Histone H4K12ac antibody (mAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 3 ug of Histone H4K12ac (mAb) or the equivalent amount of mouse IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

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  Histone H4K12ac antibody (mAb) tested by Western blot. HeLa nuclear extract (20 ug per lane) probed with Histone H4K12ac (mAb) at a 2 ug/ml dilution. Lane 1: No treatment. Lane 2: cells treated with sodium butyrate.

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  Histone H4K12ac antibody (mAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K12ac antibody (mAb) was applied at a dilution of 0.7 ug/ml to Active Motif's MODified Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.

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  Histone H4K12ac antibody (mAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H4K12ac antibody (mAb) for acetyl-Lys12 histone H4. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H4K12ac antibody (mAb) at a dilution of 2 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: acetyl-Lys5 peptide. Lane 2: unmodified Lys5 peptide. Lane 3: acetyl-Lys8 peptide. Lane 4: unmodified Lys8 peptide. Lane 5: acetyl-Lys12 peptide. Lane 6: unmodified Lys12 peptide. Lane 7: acetyl-Lys16 peptide. Lane 8: unmodified Lys16 peptide. Lane 9: acetyl-Lys20 peptide. Lane 10: unmodified Lys20 peptide. Lane 11: acetyl-Lys31 peptide. Lane 12: unmodified Lys31 peptide.

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Product Information

• Tested Applications: ChIP, DB, WB

  Applications Validated by Active Motif: ChIP: 2 - 5 ug per ChIP WB*: 0.5 - 2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Modification: Acetylated
• Immunogen: This antibody was raised against a synthetic peptide containing acetyl-lysine 12 of human Histone H4.
• Full Name: Histone H4K12ac antibody (mAb) (Clone MABI 0412)
• Synonyms: histone H4, histone, H4, histone-H4, histoneH4, mAb, monoclonal, mabi, H4 AcK12, Histone H4 Acetyl Lys12, Histone H4 Acetyl K12, Histone H4 Ac Lys12, H4 Acetyl Lys12, H4 Acetyl K12, H4 Ac Lys12, Histone H4 Acetylated Lysine 12, Histone H4 Acetyl Lysine12, H4 Acetylated Lysine 12, H4 Acetyl Lysine12, antibody, antibodies, H4K12Ac, chromatin, nucleosome, chromatin immunoprecipitation, chip, western blotting, wb
• Molecular weight: 8 kDa
• GenBank accession number: NP_778224
• RRID: AB_2793670
• Purification Method: Protein G Chromatography
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. The acetylation of histone H4 Lys12 is carried out by several histone acetyltransferases (HATs). Histone H4 Lys12 acetylation by TIP60 occurs in the nucleus, whereas Hat1-mediated acetylation of histone H4 Lys12 occurs in the cytoplasm, indicating the spatial regulation of histone acetylation. The acetylation of histone H4 Lys12 residue is commonly associated with transcriptional activation.