Histone H4 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human, Wide Range Predicted
Applications
WB
Cat No : 61199,61200 61199
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB*: 0.5 - 2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For Histone H4, we also offer AbFlex Histone H4 Recombinant Antibody (rAb). For details, see Catalog No. 91295. |
| Tested Reactivity | Human, Wide Range Predicted |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This Histone H4 antibody was raised against a full-length recombinant Xenopus Histone H4. |
| Full Name | Histone H4 antibody (pAb) |
| Synonyms | histone H4, histone, H4, histone-H4, histoneH4, pAb, polyclonal, antibody, antibodies, sample |
| Molecular weight | 8 kDa |
| GenBank accession number | NP_001087926 |
| RRID | AB_2793550 |
| Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39269) of this antibody is also available. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.