• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Histone H3S10ph antibody (mAb) (Clone MABI 0312)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Wide Range Predicted

Applications

DB, ICC, IF, WB

CloneNo.

MABI 0312

Activ Motif logo

Cat No : 39636 39636

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, mitosis, mAb, monoclonal, H3 P-S10, Histone H3 Phospho Ser10, Histone H3 Phosphorylated Ser10, Histone H3 Phosphorylated Serine 10, Histone H3 P-Ser10, Histone H3 Phospho S10, H3 Phospho Ser10, H3 P-Ser10, antibody, antibodies, MABI, clone 0312, H3S10Ph, 61373

Validation Data Gallery

  • Histone H3 phospho Ser10 mAb tested by immunofluorescence. Staining of HeLa cells with Histone H3 phospho Ser10 mAb (1 ug/ml, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

    Histone H3 phospho Ser10 mAb tested by immunofluorescence. Staining of HeLa cells with Histone H3 phospho Ser10 mAb (1 ug/ml, top panel) and DAPI (middle panel), and a merge of both images (bottom panel).

  • Histone H3S10ph antibody (mAb) tested by Western blot. HeLa nuclear extract (30 ug per lane) was probed with Histone H3S10ph antibody at a 2 ug/ml dilution. Lane 1: No treatment. Lane 2: Cells treated with colcemid.

    Histone H3S10ph antibody (mAb) tested by Western blot. HeLa nuclear extract (30 ug per lane) was probed with Histone H3S10ph antibody at a 2 ug/ml dilution. Lane 1: No treatment. Lane 2: Cells treated with colcemid.

  • Histone H3 phospho Ser10 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser10 mAb for phospho Ser10 histone H3. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: peptide phosphorylated at serine 10. Lane 2: unmodified serine 10 peptide. Lane 3: peptide phosphorylated at serine 28. Lane 4: unmodified serine 28 peptide.

    Histone H3 phospho Ser10 mAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 phospho Ser10 mAb for phospho Ser10 histone H3. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: peptide phosphorylated at serine 10. Lane 2: unmodified serine 10 peptide. Lane 3: peptide phosphorylated at serine 28. Lane 4: unmodified serine 28 peptide.

Product Information

Tested Applications DB, ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 1 ug/ml dilution WB: 0.2 - 2 ug/ml dilution DB: 1 ug/ml dilution This antibody has not been validated in ChIP, but ChIP-validated antibodies are available for this target: Catalog No. 39253. For Histone H3S10ph, we also offer AbFlex Histone H3S10ph Recombinant Antibody (rAb). For details, see Catalog No. 91131.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
ModificationPhosphorylated
Immunogen This Histone H3 phospho Ser10 antibody was raised against a peptide containing phospho serine 10 of human histone H3.
Full Name Histone H3S10ph antibody (mAb) (Clone MABI 0312)
Synonyms histone H3, histone, H3, histone-H3, histoneH3, mitosis, mAb, monoclonal, H3 P-S10, Histone H3 Phospho Ser10, Histone H3 Phosphorylated Ser10, Histone H3 Phosphorylated Serine 10, Histone H3 P-Ser10, Histone H3 Phospho S10, H3 Phospho Ser10, H3 P-Ser10, antibody, antibodies, MABI, clone 0312, H3S10Ph, 61373
Molecular weight 17 kDa
GenBank accession numberNP_003522
RRIDAB_2793285
Purification Method Protein G Chromatography
Buffer PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Interestingly, phosphorylation of Ser10 on histone H3 is involved in both transcription and cell division, two events requiring opposite alterations in the degree of chromatin compaction. Ser10 in the tail of histone H3 is phosphorylated early in mitosis when chromosomes begin to condense, and during premature chromosome condensation induced in S-phase cells.