Histone H3K56ac antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Budding Yeast, Human, Wide Range Predicted

Applications

ChIP, ChIP-Seq, DB, WB

Cat No : 39082,39281,39282

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3 AcK56, Histone H3 Acetyl Lys56, Histone H3 Acetyl K56, Histone H3 Ac Lys56, H3 Acetyl Lys56, H3 Acetyl K56, H3 Ac Lys56, Histone H3 Acetylated Lysine 56, Histone H3 Acetyl Lysine56, H3 Acetylated Lysine 56, H3 Acetyl Lysine56, antibody, antibodies, chip, chromatin immunoprecipitation, H3K56Ac, H3 K56Ac, H3AcK56, H3K56Ac, sample, chiphsabs

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Validation Data Gallery

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  Histone H3K56ac antibody (pAb) tested by ChIP-chip. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 3 million HeLa cells and 5 ul of antibody. ChIP DNA was amplified by WGA, labeled and hybridized to a human tiling array. The image on the left shows H3K56Ac binding across a 650,000 bp region on chromosome 17. The image on the right shows H3K56Ac binding at the KLF5 gene.

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  Histone H3 acetyl Lys56 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 2 ul of Histone H3 acetyl Lys56 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

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  Histone H3K56ac antibody tested by Western blot. HeLa acid extract (20 ug/lane) was probed with Histone H3 acetyl Lys56 polyclonal antibody (1:5,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.

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  Histone H3 acetyl Lys56 antibody tested by Western blot. Acid extract of yeast cells probed with Histone H3 acetyl Lys56 pAb (1:1,400 dilution).

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  Histone H3 acetyl Lys56 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 acetyl Lys56 pAb for acetyl Lys56 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:20,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: H3 acetyl-Lys4 peptide. Lane 2: H3 acetyl-Lys9 peptide. Lane 3: H3 acetyl-Lys14 peptide. Lane 4: H3 acetyl-Lys18 peptide.. Lane 5: unmodified H3 peptide aa 13-22. Lane 6: H3 acetyl-Lys23 peptide. Lane 7: H3 acetyl-Lys27 peptide. Lane 8: H3 acetyl-Lys36 peptide. Lane 9: H3 acetyl-Lys37 peptide. Lane 10: yeast H3 acetyl-Lys56 peptide. Lane 11: unmodified yeast H3 peptide aa 50-59. Lane 12: human H3 acetyl-Lys56 peptide.

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Product Information

• Tested Applications: ChIP, ChIP-Seq, DB, WB

  Applications Validated by Active Motif: ChIP: 3 - 5 ul per ChIP ChIP-Seq: 10 ul each WB: 1:2,500 - 1:5,000 dilution For Histone H3K56ac, we also offer AbFlex Histone H3K56ac Recombinant Antibody (rAb). For details, see Catalog No. 91127.

• Tested Reactivity: Budding Yeast, Human, Wide Range Predicted
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Modification: Acetylated
• Immunogen: This Histone H3 acetyl Lys56 antibody was raised against peptide containing acetyl-Lys56 of yeast histone H3.
• Full Name: Histone H3K56ac antibody (pAb)
• Synonyms: histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3 AcK56, Histone H3 Acetyl Lys56, Histone H3 Acetyl K56, Histone H3 Ac Lys56, H3 Acetyl Lys56, H3 Acetyl K56, H3 Ac Lys56, Histone H3 Acetylated Lysine 56, Histone H3 Acetyl Lysine56, H3 Acetylated Lysine 56, H3 Acetyl Lysine56, antibody, antibodies, chip, chromatin immunoprecipitation, H3K56Ac, H3 K56Ac, H3AcK56, H3K56Ac, sample, chiphsabs
• Molecular weight: 17 kDa
• GenBank accession number: NP_009564
• RRID: AB_2661786
• Purification Method: Affinity Purified
• Buffer: Purified rabbit IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histones is often associated with transcriptional activation. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.