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Histone H3K4me3 antibody (mAb) (Clone MABI 0304)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Wide Range Predicted

Applications

ChIP, ChIP-Seq, ICC, IF, WB

CloneNo.

MABI 0304

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Cat No : 61379,61979 61379

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, pAb, monoclonal, H3K4me3, Histone H3 trimethyl Lys4, Histone H3 trimethyl Lysine 4, Histone H3 trimethyl Lys 4, Histone H3 trimethyl K4, Histone H3 trimethyl K 4, Histone H3 tri-methyl Lys4, Histone H3 tri-methyl Lysine 4, Histone H3 tri-methyl Lys 4, Histone H3 tri-methyl K4, Histone H3 tri-methyl K 4, Histone H3 3Me Lys4, Histone H3 3Me Lysine 4, Histone H3 3Me Lys 4, Histone H3 3Me K4, Histone H3 3Me K 4, Histone H3 Me3 Lys4, Histone H3 Me3 Lysine 4, Histone H3 Me3 Lys 4, Histone H3 Me3 K4, Histone H3 Me3 K 4, antibody, antibodies, MABI, Clone MABI 0304, H3K4Me3, 61380

Validation Data Gallery

  • Histone H3K4me3 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 15 ug of chromatin from a human medulloblastoma cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify Histone H3K4me3 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

    Histone H3K4me3 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 15 ug of chromatin from a human medulloblastoma cell line and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify Histone H3K4me3 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

  • Histone H3 trimethyl Lys4 antibody tested by Western blot. HeLa acid extract (20 ug) probed with Histone H3K4me3 antibody (10 ug/ml dilution).

    Histone H3 trimethyl Lys4 antibody tested by Western blot. HeLa acid extract (20 ug) probed with Histone H3K4me3 antibody (10 ug/ml dilution).

Product Information

Tested Applications ChIP, ChIP-Seq, ICC, IF, WB

Applications Validated by Active Motif: ChIP: 5 - 10 ug per ChIP ChIP-Seq: 5 - 10 ug each WB: 2 - 10 ug/ml dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here. NGS-QC certification: this antibody has been processed by the NGS-QC generator. For additional details, click here. For Histone H3K4me3, we also offer AbFlex Histone H3K4me3 Recombinant Antibody (rAb). For details, see Catalog No. 91263.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
ModificationMethylated
Immunogen This Histone H3 trimethyl Lys4 antibody was raised against a synthetic peptide containing trimethyl Lys4 of human histone H3.
Full Name Histone H3K4me3 antibody (mAb) (Clone MABI 0304)
Synonyms histone H3, histone, H3, histone-H3, histoneH3, pAb, monoclonal, H3K4me3, Histone H3 trimethyl Lys4, Histone H3 trimethyl Lysine 4, Histone H3 trimethyl Lys 4, Histone H3 trimethyl K4, Histone H3 trimethyl K 4, Histone H3 tri-methyl Lys4, Histone H3 tri-methyl Lysine 4, Histone H3 tri-methyl Lys 4, Histone H3 tri-methyl K4, Histone H3 tri-methyl K 4, Histone H3 3Me Lys4, Histone H3 3Me Lysine 4, Histone H3 3Me Lys 4, Histone H3 3Me K4, Histone H3 3Me K 4, Histone H3 Me3 Lys4, Histone H3 Me3 Lysine 4, Histone H3 Me3 Lys 4, Histone H3 Me3 K4, Histone H3 Me3 K 4, antibody, antibodies, MABI, Clone MABI 0304, H3K4Me3, 61380
Molecular weight 17 kDa
GenBank accession numberNP_003522
RRIDAB_2793611
Purification Method Protein G Chromatography
Buffer PBS pH 7.5 containing 30% glycerol. 0.3M NaCl, and 0.035% sodium azide. Sodium azide is highly toxic..
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.