Histone H3K4me1 antibody (mAb) (Clone MABI 0302)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Wide Range Predicted

Applications

ChIP, ChIP-Seq, DB, WB

CloneNo.

MABI 0302

Cat No : 39635

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, pAb, monoclonal, H3K4me1, Histone H3 monomethyl Lys4, Histone H3 monomethyl Lysine 4, Histone H3 monomethyl Lys 4, Histone H3 monomethyl K4, Histone H3 monomethyl K 4, Histone H3 mono-methyl Lys4, Histone H3 mono-methyl Lysine 4, Histone H3 mono-methyl Lys 4, Histone H3 mono-methyl K4, Histone H3 mono-methyl K 4, Histone H3 1Me Lys4, Histone H3 1Me Lysine 4, Histone H3 1Me Lys 4, Histone H3 1Me K4, Histone H3 1Me K 4, Histone H3 Me1 Lys4, Histone H3 Me1 Lysine 4, Histone H3 Me1 Lys 4, Histone H3 Me1 K4, Histone H3 Me1 K 4, antibody, antibodies, MABI, clone 0302, chip, chromatin immunoprecipitation, H3K4Me1, 61314

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Validation Data Gallery

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  Histone H3K4me1 antibody (mAb) tested by ChIP-Seq ChIP was performed using 10 ug of chromatin from MCF7 cells and 4 ug of antibody. ChIP DNA was sequenced and 19 million sequence tags were mapped to identify Histone H3K4me1 binding sites.

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  Histone H3 monomethyl Lys4 antibody (mAb) tested by Western blot. HeLa acid extract probed with Histone H3 monomethyl Lys4 antibody (mAb) (2 ug/ml dilution).

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  Histone H3 monomethyl Lys4 antibody (mAb) tested by Dot Blot Dot Blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys4 antibody (mAb) for monomethyl Lys4 histone H3. Methylated peptides corresponding to the immunogen or related peptides were spotted onto PVDF and probed with antibody at 1 ug/ml. Lane 1: unmodified peptide, Lane 2: Monomethyl Lys4, Lanes 3-8: various negative control peptides.

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Product Information

• Tested Applications: ChIP, ChIP-Seq, DB, WB

  Applications Validated by Active Motif: ChIP-Seq: 4 ug each ChIP: 10 ul per ChIP WB: 1:500 - 1:2,000 dilution For Histone H3K4me1, we also offer AbFlex Histone H3K4me1 Recombinant Antibody (rAb). For details, see Catalog No. 91289.

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Modification: Methylated
• Immunogen: The Histone H3 monomethyl Lys4 antibody was raised against a peptide containing monomethyl lysine 4 of human histone H3.
• Full Name: Histone H3K4me1 antibody (mAb) (Clone MABI 0302)
• Synonyms: histone H3, histone, H3, histone-H3, histoneH3, pAb, monoclonal, H3K4me1, Histone H3 monomethyl Lys4, Histone H3 monomethyl Lysine 4, Histone H3 monomethyl Lys 4, Histone H3 monomethyl K4, Histone H3 monomethyl K 4, Histone H3 mono-methyl Lys4, Histone H3 mono-methyl Lysine 4, Histone H3 mono-methyl Lys 4, Histone H3 mono-methyl K4, Histone H3 mono-methyl K 4, Histone H3 1Me Lys4, Histone H3 1Me Lysine 4, Histone H3 1Me Lys 4, Histone H3 1Me K4, Histone H3 1Me K 4, Histone H3 Me1 Lys4, Histone H3 Me1 Lysine 4, Histone H3 Me1 Lys 4, Histone H3 Me1 K4, Histone H3 Me1 K 4, antibody, antibodies, MABI, clone 0302, chip, chromatin immunoprecipitation, H3K4Me1, 61314
• Molecular weight: 17 kDa
• GenBank accession number: NP_003522
• RRID: AB_2793284
• Purification Method: Protein G Chromatography
• Buffer: PBS pH 7.5 containing 30% glycerol. 0.3M NaCl, and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.