Histone H3K36me2 antibody (mAb) (Clone MABI 0332)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Wide Range Predicted

Applications

ChIP, DB, IF, WB

CloneNo.

MABI 0332

Cat No : 61019

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, mAb, monoclonal, H3K36me2, Histone H3 dimethyl Lys36, Histone H3 dimethyl Lysine 36, Histone H3 dimethyl Lys 36, Histone H3 dimethyl K36, Histone H3 dimethyl K 36, Histone H3 di-methyl Lys36, Histone H3 di-methyl Lysine 36, Histone H3 di-methyl Lys 36, Histone H3 di-methyl K36, Histone H3 di-methyl K 36, Histone H3 2Me Lys36, Histone H3 2Me Lysine 36, Histone H3 2Me Lys 36, Histone H3 2Me K36, Histone H3 2Me K 36, Histone H3 Me2 Lys36, Histone H3 Me2 Lysine 36, Histone H3 Me2 Lys 36, Histone H3 Me2 K36, Histone H3 Me2 K 36, antibody, antibodies, H3K36Me2, Clone MABI 0332, 61320

---

Validation Data Gallery

• 

  Western blot of Histone H3 dimethyl Lys36 antibody. HeLa nuclear extract (20 ug per lane) probed with H3K36me2 antibody (2 ug/ml dilution).

• 

  Dot blot of Histone Histone H3 dimethyl Lys36 antibody. Dot blot analysis was used to confirm the specificity of H3K36me2 antibody for dimethyl Lys36 of histone H3. Recombinant methylated peptides corresponding to the immunogen and related sequences were spotted onto PVDF and probed with H3K36me2 at 2 ug/ml. The amount of protein (picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified H3K4. Lane 2: H3K4me1. Lane 3: H3K4me2. Lane 4: H3K4me3. Lane 5: unmodified H3K9. Lane 6: H3K9me1. Lane 7: H3K9me2. Lane 8: H3K9me3. Lane 9: unmodified H3K79. Lane 10: H3K79me1. Lane 11: H3K79me2. Lane 12: H3K79me3. Bottom panel - Lane 1: unmodified H3K23. Lane 2: H3K23me1. Lane 3: H3K23me3. Lane 4: H3K23me3. Lane 5: unmodified H3K27. Lane 6: H3K27me1. Lane 7: H3K27me2. Lane 8: H3K27me3. Lane 9: unmodified H3K36. Lane 10: H3K36me1. Lane 11: H3K36me2. Lane 12: H3K36me3.

---

Product Information

• Tested Applications: ChIP, DB, IF, WB

  Applications Validated by Active Motif: WB: 0.5 - 2 ug/ml dilution DB: 0.5 - 2 ug/ml dilution

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Modification: Methylated
• Immunogen: This Histone H3 dimethyl Lys36 antibody was raised against a peptide containing dimethyl Lys36 of human Histone H3.
• Full Name: Histone H3K36me2 antibody (mAb) (Clone MABI 0332)
• Synonyms: histone H3, histone, H3, histone-H3, histoneH3, mAb, monoclonal, H3K36me2, Histone H3 dimethyl Lys36, Histone H3 dimethyl Lysine 36, Histone H3 dimethyl Lys 36, Histone H3 dimethyl K36, Histone H3 dimethyl K 36, Histone H3 di-methyl Lys36, Histone H3 di-methyl Lysine 36, Histone H3 di-methyl Lys 36, Histone H3 di-methyl K36, Histone H3 di-methyl K 36, Histone H3 2Me Lys36, Histone H3 2Me Lysine 36, Histone H3 2Me Lys 36, Histone H3 2Me K36, Histone H3 2Me K 36, Histone H3 Me2 Lys36, Histone H3 Me2 Lysine 36, Histone H3 Me2 Lys 36, Histone H3 Me2 K36, Histone H3 Me2 K 36, antibody, antibodies, H3K36Me2, Clone MABI 0332, 61320
• Molecular weight: 17 kDa
• GenBank accession number: NP_003522
• RRID: AB_2650523
• Purification Method: Protein G Chromatography
• Buffer: PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.