Histone H3K27me2 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human, Wide Range Predicted
Applications
DB, ICC, IF, WB
Cat No : 39919,39920 39919
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
DB, ICC, IF, WB
Applications Validated by Active Motif: ICC/IF: 1 - 2 ug/ml dilution WB*: 1 - 2 ug/ml dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. |
| Tested Reactivity | Human, Wide Range Predicted |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Modification | Methylated |
| Immunogen | This Histone H3 dimethyl Lys27 antibody was raised against a peptide including dimethyl-lysine 27 of histone H3. |
| Full Name | Histone H3K27me2 antibody (pAb) |
| Synonyms | histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3K27me2, Histone H3 dimethyl Lys27, Histone H3 dimethyl Lysine 27, Histone H3 dimethyl Lys 27, Histone H3 dimethyl K27, Histone H3 dimethyl K 27, Histone H3 di-methyl Lys27, Histone H3 di-methyl Lysine 27, Histone H3 di-methyl Lys 27, Histone H3 di-methyl K27, Histone H3 di-methyl K 27, Histone H3 2Me Lys27, Histone H3 2Me Lysine 27, Histone H3 2Me Lys 27, Histone H3 2Me K27, Histone H3 2Me K 27, Histone H3 Me2 Lys27, Histone H3 Me2 Lysine 27, Histone H3 Me2 Lys 27, Histone H3 Me2 K27, Histone H3 Me2 K 27, antibody, antibodies, H3K27Me2, polycomb, stem, sample |
| Molecular weight | 17 kDa |
| GenBank accession number | NP_003522 |
| RRID | AB_2793393 |
| Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39245) of this antibody is also available. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.