Histone H3K27ac antibody (pAb)
Host / Isotype
Rabbit / Serum
Reactivity
Budding Yeast, Human, Wide Range Predicted
Applications
ChIP, ChIP-Seq, CUT&Tag, DB, WB
Cat No : 39135,39136,39336 39135
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
ChIP, ChIP-Seq, CUT&Tag, DB, WB
Applications Validated by Active Motif: ChIP: 3 ul per ChIP ChIP-Seq: 5 ul each WB*: 1:1,000 - 1:5,000 dilution CUT&Tag: 1 ul per 50 ul reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For Histone H3K27ac, we also offer AbFlex Histone H3K27ac Recombinant Antibody (rAb). For details, see Catalog No. 91193. |
| Tested Reactivity | Budding Yeast, Human, Wide Range Predicted |
| Host / Isotype | Rabbit / Serum |
| Class | Polyclonal |
| Type | Antibody |
| Modification | Acetylated |
| Immunogen | This Histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3. |
| Full Name | Histone H3K27ac antibody (pAb) |
| Synonyms | histone H3, histone, H3, histone-H3, histoneH3, pAb, polyclonal, H3 AcK27, Histone H3 Acetyl Lys27, Histone H3 Acetyl K27, Histone H3 Ac Lys27, H3 Acetyl Lys27, H3 Acetyl K27, H3 Ac Lys27, Histone H3 Acetylated Lysine 27, Histone H3 Acetyl Lysine27, H3 Acetylated Lysine 27, H3 Acetyl Lysine27, antibody, antibodies, chip, chromatin immunoprecipitation, H3K27Ac, sample |
| Molecular weight | 17 kDa |
| GenBank accession number | NP_003522 |
| RRID | AB_2614979 |
| Purification Method | None |
| Buffer | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39133) of this antibody that was purified by Protein A Chromatography is also available. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histone H3 at Lys27 is associated with transcriptional activation.Histone H3K27 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.