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Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Wide Range Predicted

Applications

ChIP, ChIP-Seq, ICC, IF, WB

CloneNo.

1D4F2

Activ Motif logo

Cat No : 61629,61630 61629

Synonyms

Histone H3.1 / 3.2, 3.1, 3.2, Histone H3, globular domain, nucleosome, chromatin, antibody, antibodies, monoclonal, histone H3, histone, H3, histone-H3, histoneH3, mAb, antibody, antibodies, chip, chromatin immunoprecipitation, chip-seq, immunofluorescence, if, immunocytochemistry, icc, western blotting, wb, sample, h3variant

Validation Data Gallery

  • Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by immunofluorescence. Top: HeLa cell stained with H3.1 / 3.2 antibody (mAb). Bottom: Hoechst.

    Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by immunofluorescence. Top: HeLa cell stained with H3.1 / 3.2 antibody (mAb). Bottom: Hoechst.

  • Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by Western blot. HeLa nuclear extract (20 ug) and recombinant human Histones (100 ng) were probed with Histone H3.1 / 3.2 antibody (mAb) at a 1 ug/ml dilution in lanes 1, 2, & 3. Histone H3 (mAb) (Catalog No. 61475) is also shown at a 0.25 ug/ml dilution in lanes 4 & 5. Lane 1: Nuclear extract of untreated HeLa cells. Lane 2: 100 ng recombinant human Histone H3.1 protein. Lane 3: 100 ng recombinant human Histone H3.3 protein. Lane 4: 100 ng recombinant human Histone H3.1 protein. Lane 5: 100 ng recombinant human Histone H3.3 protein.

    Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2) tested by Western blot. HeLa nuclear extract (20 ug) and recombinant human Histones (100 ng) were probed with Histone H3.1 / 3.2 antibody (mAb) at a 1 ug/ml dilution in lanes 1, 2, & 3. Histone H3 (mAb) (Catalog No. 61475) is also shown at a 0.25 ug/ml dilution in lanes 4 & 5. Lane 1: Nuclear extract of untreated HeLa cells. Lane 2: 100 ng recombinant human Histone H3.1 protein. Lane 3: 100 ng recombinant human Histone H3.3 protein. Lane 4: 100 ng recombinant human Histone H3.1 protein. Lane 5: 100 ng recombinant human Histone H3.3 protein.

Product Information

Tested Applications ChIP, ChIP-Seq, ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 0.4 - 2 ug/ml dilution WB*: 0.2 - 2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against a peptide comprising amino acids 21-39 of human Histone H3.1. This region is 100% identical in human Histone H3.2.
Full Name Histone H3.1 / 3.2 antibody (mAb) (Clone 1D4F2)
Synonyms Histone H3.1 / 3.2, 3.1, 3.2, Histone H3, globular domain, nucleosome, chromatin, antibody, antibodies, monoclonal, histone H3, histone, H3, histone-H3, histoneH3, mAb, antibody, antibodies, chip, chromatin immunoprecipitation, chip-seq, immunofluorescence, if, immunocytochemistry, icc, western blotting, wb, sample, h3variant
Molecular weight 17 kDa
GenBank accession numberNP_003522
RRIDAB_2793710
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.2 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.2 has a serine.