Histone H2B antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB
Cat No : 39210 39210
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 2 ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required. |
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This Histone H2B antibody was raised against a peptide corresponding to amino acid residues 111-125 of human Histone H2B. |
| Full Name | Histone H2B antibody (pAb) |
| Synonyms | histone H2B, histone, H2B, histone-H2B, histoneH2B, pAb, polyclonal, antibody, antibodies |
| Molecular weight | 15 kDa |
| GenBank accession number | NP_778225 |
| RRID | AB_2793185 |
| Purification Method | Protein G Chromatography |
| Buffer | PBS containing 0.05% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.