Histone H2A.X antibody (pAb) (H2AX)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, ICC, IF, WB

Cat No : 39689,39690

Synonyms

histone H2A.X, histone H2AX, histone, H2A.X, H2AX, histone-H2AX, histone-H2A.X, histoneH2AX, histoneH2A.X, gammaH2AX, gammah2A.X, gamma-h2ax, gamma-h2a.x, gamma h2ax, gamma h2A.X, pAb, polyclonal, H2AX P-S139, H2A histone family member X, Histone H2 Phospho Ser139, Histone H2 Phosphorylated Ser139, Histone H2 Phosphorylated Serine 139, Histone H2 P-Ser139, Histone H2 Phospho S139, H2 Phospho Ser139, H2 P-Ser139, antibody, antibodies, hisvar, sample

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Validation Data Gallery

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  Histone H2AX antibody tested by immunofluorescence. Detection of Histone H2AX by immunofluorescence. HeLa cells were stained with Histone H2AX antibody at a dilution of 1:1,000. Top panel: Histone H2AX antibody staining. Middle panel: DAPI. Bottom panel: merge.

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  Histone H2AX pAb tested by Western blot. Western blot probed with Histone H2AX pAb at a dilution of 1:2,000. Lane 1: Recombinant human histone H2AX (100 ng). Lane 2: Mouse embryonic fibroblast (MEF) cell wild type nuclear lysate. Lane 3: MEF cell nuclear extract derived from H2AX -/- nulls.

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  Histone H2AX pAb tested by Western blot. 30 ug of HeLa cell nuclear extract was probed with Histone H2AX pAb at a dilution of 1:2,000.

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Product Information

• Tested Applications: ChIP, ICC, IF, WB

  Applications Validated by Active Motif: ICC/IF: 1:500 - 1:1,000 dilution WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This Histone H2AX antibody was raised against a peptide derived from the C-terminus of human histone H2AX.
• Full Name: Histone H2A.X antibody (pAb) (H2AX)
• Synonyms: histone H2A.X, histone H2AX, histone, H2A.X, H2AX, histone-H2AX, histone-H2A.X, histoneH2AX, histoneH2A.X, gammaH2AX, gammah2A.X, gamma-h2ax, gamma-h2a.x, gamma h2ax, gamma h2A.X, pAb, polyclonal, H2AX P-S139, H2A histone family member X, Histone H2 Phospho Ser139, Histone H2 Phosphorylated Ser139, Histone H2 Phosphorylated Serine 139, Histone H2 P-Ser139, Histone H2 Phospho S139, H2 Phospho Ser139, H2 P-Ser139, antibody, antibodies, hisvar, sample
• Molecular weight: 15 kDa
• GenBank accession number: NP_002096
• RRID: AB_2728764
• Purification Method: Affinity Purified
• Buffer: Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H2AX (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation.