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Histone H2A.J antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

DB, WB

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Cat No : 61793,61794 61793

Synonyms

histone H2A, H2, histone, histones, H2A, histone-H2A, histoneH2A, pAb, polyclonal, antibody, antibodies

Validation Data Gallery

  • Western blot of Histone H2A.J pAb. 20 ug Nuclear Extract of HeLa cells (Lane 1) or 40 ug mouse 3T3-L1 cells before (Lane 2) or following stimulation for 12 days with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) resulting in differentiation into adipocytes (Lane 3). MW:14kD

    Western blot of Histone H2A.J pAb. 20 ug Nuclear Extract of HeLa cells (Lane 1) or 40 ug mouse 3T3-L1 cells before (Lane 2) or following stimulation for 12 days with a defined hormonal mixture (DMSO, dexamethasone, insulin, and IBMX) resulting in differentiation into adipocytes (Lane 3). MW:14kD

  • Dot blot of Histone H2A.J pAb. Dot blot analysis was used to confirm the specificity of Histone H2A.J antibody. Lane 1: H2A.J peptide. Lane 2: H2A peptide comprised of a.a. 114-126 of human Histone H2A.

    Dot blot of Histone H2A.J pAb. Dot blot analysis was used to confirm the specificity of Histone H2A.J antibody. Lane 1: H2A.J peptide. Lane 2: H2A peptide comprised of a.a. 114-126 of human Histone H2A.

Product Information

Tested Applications DB, WB

Applications Validated by Active Motif: WB*: 1:500 - 1:2500 dilution DB: 1:1000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide comprising amino acids 122-129 of human Histone H2A.J.
Full Name Histone H2A.J antibody (pAb)
Synonyms histone H2A, H2, histone, histones, H2A, histone-H2A, histoneH2A, pAb, polyclonal, antibody, antibodies
Molecular weight
GenBank accession numberNP_808760
RRIDAB_2793769
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, and is responsible for establishing higher-order chromatin structure. Histone H2A.J is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. Histone H2A.J, which is found only in mammals, is shown to be associated with senescence, accumulating in senescent fibroblasts and promoting expression of inflammatory genes. Therefore, H2A.J may contribute to chronic inflammation and age-related diseases.