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Histone H2A, C-terminal antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Wide Range Predicted

Applications

IF, WB

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Cat No : 39591,39592 39591

Synonyms

histone H2A, H2, histone, histones, H2A, histone-H2A, histoneH2A, c-terminal, pAb, polyclonal, antibody, antibodies, sample

Validation Data Gallery

  • Histone H2A, C-terminal pAb tested by Western blot. Detection of Histone H2A by Western blot. The analysis was performed using HeLa acid extract (10 ug, lane 1) and recombinant histone H2A protein (200 ng, lane 2) probed with Histone H2A, C-terminal pAb at a 1:500 dilution).

    Histone H2A, C-terminal pAb tested by Western blot. Detection of Histone H2A by Western blot. The analysis was performed using HeLa acid extract (10 ug, lane 1) and recombinant histone H2A protein (200 ng, lane 2) probed with Histone H2A, C-terminal pAb at a 1:500 dilution).

  • Detection of H2A by immunofluorescence.(/b> U2OS cells were stained with H2A antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2A antibody staining. Right panel: merge.

    Detection of H2A by immunofluorescence.(/b> U2OS cells were stained with H2A antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2A antibody staining. Right panel: merge.

Product Information

Tested Applications IF, WB

Applications Validated by Active Motif: IF: 1:500 dilution WB: 1:500 - 1:1,000 dilution
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This Histone H2A, C-terminal antibody was raised against a peptide containing the C- terminal region of human histone H2A.
Full Name Histone H2A, C-terminal antibody (pAb)
Synonyms histone H2A, H2, histone, histones, H2A, histone-H2A, histoneH2A, c-terminal, pAb, polyclonal, antibody, antibodies, sample
Molecular weight 14 kDa
GenBank accession numberNP_003508
RRIDAB_2793270
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression.