Histone Acetyltransferase Assay Kit (Fluorescent)

Synonyms

histone, histone acetyltransferase, acetyltransferase, acetylation, histone acetylation, histone modification, hat, HAT, hat assay, HAT assay, fluorescent, p300

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Overview

The HAT Assay Kit is a fluorescent method to assay samples for histone acetyltransferase (HAT) activity or to screen HAT inhibitors. The assay is easy to use, features a simple protocol that gives you results in 30 minutes and is ideal for high-throughput usage in 96-well plates.

Please refer to our Recombinant, full-length p300 protein, Catalog No. 81158, recently launched for use in acetyltransferase assays.

Data

The HAT Assay Kit includes enough recombinant human p300 to screen 96 wells of inhibitor compounds, and anacardic acid is included for use as a control, as it is a potent HAT inhibitor. All assay buffers have been thoroughly tested to ensure that no components interfere with either activity or inhibition assays. Histone H3 and Histone H4 N-terminal peptides are included as HAT substrates, so you can use the one best suited for your HAT enzyme.

Figure 1: p300 activity and inhibition.The HAT activity of 50 ng of p300 was assayed with 50 µM acetyl-CoA and either 50 µM Histone H3 or Histone H4 peptide as the substrate. Activity was inhibited with 15 µM anacardic acid or 25 µM garcinol.

Contents

Contents & Storage

1 x 96 rxns of recombinant p300 protein, catalytic domain; histone H3 and H4 substrate peptides, Acetyl-CoA, 5X Assay Buffer, Developer and Developing & Stop Solutions. A 96-well plate and plate sealer are also included. Recombinant p300 protein, catalytic domain; and Acetyl-CoA are to be stored at -80°C. Store histone H3 and H4 substrate peptides, 5X Assay Buffer, Developer and Developing Solution at -20°C. Stop Solution, the 96-well plate and plate sealer may be stored at room temperature. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.

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