Hep G2 whole-cell extract

Synonyms

Hep G2, HepG2, whole-cell extract, whole-cell, whole cell, extract, lysate, whole cell lysate, cell lysate, liver, liver cancer, hepatocyte, HBV, hepatitis, hepatitis B, polarization, polar, trafficking, localization, subcellular localization, liver metabolism, sigaling, signalling, signaling pathway, signalling pathway, apoptosis, cell death, cell survival, liver toxicity, liver regeneration, hypoxia, 36412

---

Contents

2 x 100 µg of Hep G2 whole-cell extract at 2.5 µg/µl.

Background

Hep G2 whole-cell extract was prepared from cell cultures of the human hepatocellular liver carcinoma Hep G2 cell line. These cells are epithelial in morphology and can be induced to exhibit polarization under appropriate culture conditions. Hep G2 cells are most commonly used for studies of hepatocyte function. Because of their ability to differentiate into polarized epithelium, these cells are routinely utilized to investigate intracellular protein trafficking, particularly in relation to human liver diseases. Hep G2 is the most commonly used cell line in studies pertaining to the regulation of hepatic protein synthesis, particularly the synthesis of acute phase proteins after the onset of a systemic inflammatory response. In addition, these cells are important for studies related to liver metabolism, liver cancers, liver regeneration, liver cytotoxicity, apoptosis and as a model for hepatitis B virus (HBV) viral etiology.

Application Notes

Hep G2 whole-cell extract is specifically recommended for studies related to 1) human liver diseases, cancers and drug therapies, 2) HBV etiology, 3) signaling pathways, and 4) apoptosis.

Extract Origin

Human Liver (hepatocellular carcinoma)

Extract Composition

Hep G2 whole-cell extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl₂, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot.

---