Hep G2 nuclear extract

Synonyms

Hep G2, HepG2, nuclear extract, nuclear, extract, lysate, nuclear lysate, liver, liver cancer, hepatocyte, HBV, hepatitis, hepatitis B, polarization, polar, trafficking, localization, subcellular localization, liver metabolism, sigaling, signalling, signaling pathway, signalling pathway, apoptosis, cell death, cell survival, liver toxicity, liver regeneration, hypoxia, 36011

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Contents

2 x 100 µg of Hep G2 nuclear extract at 2.5 µg/µl.

Background

Hep G2 nuclear extract was prepared from cell cultures of the human hepatocellular liver carcinoma Hep G2 cell line. These cells are epithelial in morphology and can be induced to form polarized epithelium under appropriate culture conditions. Hep G2 cells are most commonly used for studies related to hepatocyte function. Because of their ability to differentiate into polarized epithelium, these cells are often used to investigate intracellular protein trafficking, specifically in relation to human liver diseases. Hep G2 is the most commonly used cell line for studying the regulation of hepatic protein synthesis, particularly the synthesis of acute phase proteins after the onset of a systemic inflammatory response. In addition, these cells are used to study liver metabolism, liver cancers, liver regeneration, liver cytotoxicity, apoptosis and as a model for hepatitis B virus (HBV) viral etiology.

Application Notes

Hep G2 nuclear extract is specifically recommended for studies related to 1) human liver diseases, cancers and drug therapies, 2) HBV etiology, 3) signaling pathways, and 4) apoptosis.

Extract Origin

Human Liver (hepatocellular carcinoma)

Extract Composition

Hep G2 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl₂, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and TransAM^® HNF Family Kits.

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