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Hbo1 antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human, Wide Range Predicted

Applications

WB

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Cat No : 39105,39106 39105

Synonyms

Hbo1, Hbo-1, Hbo 1, pAb, polyclonal, antibody, antibodies, sample

Validation Data Gallery

  • Hbo1 pAb tested by Western blot. Detection of Hbo1 by Western blot analysis. HeLa whole-cell extract (15 ug) was probed with Hbo1 pAb at a 1:500 dilution.

    Hbo1 pAb tested by Western blot. Detection of Hbo1 by Western blot analysis. HeLa whole-cell extract (15 ug) was probed with Hbo1 pAb at a 1:500 dilution.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB*: 1:500 - 1:2,000 dilution For optimal results, primary antibody incubations should be performed overnight at 4°C. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
Immunogen This Hbo1 antibody was raised against a synthetic peptide containing amino acids 158-172 of human Hbo1.
Full Name Hbo1 antibody (pAb)
Synonyms Hbo1, Hbo-1, Hbo 1, pAb, polyclonal, antibody, antibodies, sample
Molecular weight 75 kDa
GenBank accession numberNP_008998
RRIDAB_2793156
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Hbo1 (HAT binding to ORC1) is a MYST-family histone acetyltransferase (HAT) that forms a complex with ING4/5, JADE1/2/3, and Eaf6, which is found enriched on coding regions of genes, supporting a role in transcriptional elongation. In addition, Hbo1 is thought to participate in events controlling the initiation of DNA replication.