HNF4A antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ICC, IF, WB

Cat No : 61189,61190

Synonyms

HNF4A, hepatocyte nuclear receptor 4A, NR2A1, TCF14, orphan nuclear receptor, dbdabs, hepatocyte, lipid homeostasis, maturity onset diabetes of the young, MODY1, liver, kidney, intestine, sample

---

Validation Data Gallery

• 

  HNF4A antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with HNF4A antibody at a 1:2,000 dilution.

• 

  HNF4A antibody (pAb) tested by Western blot. Whole cell extract (20 ug) of Hep G2 cells probed with HNF4A antibody at a dilution of 1:500.

---

Product Information

• Tested Applications: ICC, IF, WB

  Applications Validated by Active Motif: ICC/IF: 1:2,000 dilution WB: 1:500 - 1:2,000 dilution For optimal results, primary antibody incubations should be performed at 4°C. Individual optimization may be required.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This HNF4A antibody was raised against a peptide derived from the N-terminal region of human HNF4A.
• Full Name: HNF4A antibody (pAb)
• Synonyms: HNF4A, hepatocyte nuclear receptor 4A, NR2A1, TCF14, orphan nuclear receptor, dbdabs, hepatocyte, lipid homeostasis, maturity onset diabetes of the young, MODY1, liver, kidney, intestine, sample
• Molecular weight: 60 kDa
• GenBank accession number: NP_000448
• RRID: AB_2793545
• Purification Method: Affinity Purified
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

HNF4A (hepatocyte nuclear receptor 4A, NR2A1, TCF14) is an orphan nuclear receptor and activator of transcription. HNF4A is major regulator of the hepatocytic phenotype and regulates genes involved in the control of lipid homeostasis. Mutations in HNF4A can cause maturity onset diabetes of the young (MODY1). HNF4A binds DNA as a homodimer and is involved in the development of the liver, kidney and intestine.