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HIF-1 alpha antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human

Applications

ChIP, ChIP-Seq, WB

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Cat No : 39665,39666 39665

Synonyms

HIF-1 alpha, Hif1a, Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor), MOP1, HIF1, Basic-Helix-Loop-Helix-PAS Protein MOP1, HIF1-ALPHA, Class E Basic Helix-Loop-Helix Protein 78, ARNT Interacting Protein, Member Of PAS Protein 1, Hypoxia-Inducible Factor 1 Alpha Isoform I.3, HIF-1-alpha, Hypoxia-Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor), PASD8, Hypoxia-Inducible Factor 1-Alpha, ARNT-Interacting Protein, Hypoxia-Inducible Factor1alpha, Member Of PAS Superfamily 1, PAS Domain-Containing Protein 8, HIF-1A, HIF1-alpha, oxygen, transcription factor, polyclonal, antibody, antibodies, samples, western blotting, wb

Validation Data Gallery

  • HIF-1 alpha antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 5 ul of HIF-1 alpha antibody and 25 ug of chromatin from hypoxic Huh7 cells. ChIP DNA was sequenced on the Illumina HiSeq and 19 million sequence tags were mapped to identify Hif-1 alpha binding sites across the genome. The images show binding in the promoters of Hif1a target genes that regulate metabolism.

    HIF-1 alpha antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 5 ul of HIF-1 alpha antibody and 25 ug of chromatin from hypoxic Huh7 cells. ChIP DNA was sequenced on the Illumina HiSeq and 19 million sequence tags were mapped to identify Hif-1 alpha binding sites across the genome. The images show binding in the promoters of Hif1a target genes that regulate metabolism.

  • HIF-1 alpha antibody (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 5 ul of HIF-1 alpha antibody and 25 ug of chromatin from hypoxic Huh7 cells. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

    HIF-1 alpha antibody (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 5 ul of HIF-1 alpha antibody and 25 ug of chromatin from hypoxic Huh7 cells. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

  • HIF-1 alpha antibody (pAb) tested by Western blot. Detection of HIF-1 alpha by Western blot analysis with 20 ug COS-7 nuclear extract either untreated (lane 1) or treated with CoCl2 (lane 2).

    HIF-1 alpha antibody (pAb) tested by Western blot. Detection of HIF-1 alpha by Western blot analysis with 20 ug COS-7 nuclear extract either untreated (lane 1) or treated with CoCl2 (lane 2).

Product Information

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP: 5 ul per ChIP ChIP-Seq: 5 ul per ChIP-Seq WB: 1:500 - 1:1,000 dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. ChIP-validated antibodies are available for this target: Catalog No. 39253. For HIF-1 alpha, we also offer AbFlex HIF-1 alpha Recombinant Antibody (rAb). For details, see Catalog No. 91139.
Tested Reactivity Human
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
Immunogen This HIF-1 alpha antibody was raised against a synthetic peptide corresponding to the C-terminal region of human HIF-1 alpha.
Full Name HIF-1 alpha antibody (pAb)
Synonyms HIF-1 alpha, Hif1a, Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor), MOP1, HIF1, Basic-Helix-Loop-Helix-PAS Protein MOP1, HIF1-ALPHA, Class E Basic Helix-Loop-Helix Protein 78, ARNT Interacting Protein, Member Of PAS Protein 1, Hypoxia-Inducible Factor 1 Alpha Isoform I.3, HIF-1-alpha, Hypoxia-Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor), PASD8, Hypoxia-Inducible Factor 1-Alpha, ARNT-Interacting Protein, Hypoxia-Inducible Factor1alpha, Member Of PAS Superfamily 1, PAS Domain-Containing Protein 8, HIF-1A, HIF1-alpha, oxygen, transcription factor, polyclonal, antibody, antibodies, samples, western blotting, wb
Molecular weight 100 kDa
GenBank accession numberNP_001521
RRIDAB_2614934
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Hypoxia-inducible factor-1 (HIF1) is a transcription factor involved in the regulation of oxygen homeostasis. It regulates the physiological responses to low oxygen levels (hypoxia) and the pathophysiology of heart attack, cancer, stroke and chronic lung disease. HIF-1 is a heterodimeric protein that consists of two subunits, HIF-1 alpha and HIF-1 beta (also known as ARNT1, the aryl hydrocarbon nuclear translocator). While HIF-1 beta is constitutively expressed, the expression of HIF-1 alpha is induced by oxygen concentrations below 6%. Heterodimerization is required to bind the hypoxia response element (HRE).