HIF-1 alpha antibody (mAb) (Clone ESEE122)
Host / Isotype
Mouse / IgG1
Reactivity
Bovine, Human, Mouse, Rat
Applications
IHC, WB
CloneNo.
ESEE122
Cat No : 61275,61276 61275
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
IHC, WB
This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91139. |
| Tested Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This HIF-1 alpha antibody was raised against a partial protein containing amino acid residues 329-530 of human HIF-1 alpha. |
| Full Name | HIF-1 alpha antibody (mAb) (Clone ESEE122) |
| Synonyms | HIF-1 alpha, hypoxia inducible factor 1, alpha subunit, MOP1, PASD8, bHLHe78, PAS domain-containing protein 8, HIF1, monoclonal, antibody, mAb, sample, Immunohistochemistry, IHC, transcription, oxidative stress |
| Molecular weight | 100 kDa |
| GenBank accession number | NP_001521 |
| RRID | AB_2615045 |
| Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
HIF-1 (hypoxia inducible factor 1, alpha subunit, MOP1, PASD8, bHLHe78, PAS domain-containing protein 8) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.