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Geminin antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human

Applications

IF, IP, WB

Activ Motif logo

Cat No : 61135,61136 61135

Synonyms

Geminin, Gemi, GMNN, centrosome, DNA replication, MCM complex, CDT1, licensing, embryonic stem cell, esc, pluripotency, pluripotent, cell cycle, sample

Validation Data Gallery

  • Detection of Geminin by immunofluorescence U2OS cells were stained with Geminin antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: Geminin antibody staining. Right panel: merge.

    Detection of Geminin by immunofluorescence U2OS cells were stained with Geminin antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: Geminin antibody staining. Right panel: merge.

Product Information

Tested Applications IF, IP, WB

Applications Validated by Active Motif: IF: 1:500 dilution
Tested Reactivity Human
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
Immunogen This Geminin antibody was raised against full-length recombinant human Geminin protein.
Full Name Geminin antibody (pAb)
Synonyms Geminin, Gemi, GMNN, centrosome, DNA replication, MCM complex, CDT1, licensing, embryonic stem cell, esc, pluripotency, pluripotent, cell cycle, sample
Molecular weight 33 kDa
GenBank accession numberNP_056979
RRIDAB_2793521
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Geminin is a crucial cell cycle regulatory protein that controls DNA replication and centrosome function. Geminin inhibits DNA replication by preventing the binding of the MCM complex into the pre-replication complex. Geminin accumulates during S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin functions as a homotetramer and interacts with DNA replication licensing factor CDT1. The interaction between Geminin and CDT1 is crucial to restrict DNA replication to once per cell cycle. Geminin is required for the formation of pluripotent cells in the developing embryo and essential for pluripotency in the embryonic stem cells. In ESCs, Geminin remains during G1 and sustains core pluripotency factors (Oct4, Sox2, and Nanog) by antagonizing the action of the chromatin remodeling protein BRG1.