GLI1 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB
Cat No : 61215,61216 61215
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. |
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This GLI1 antibody was raised against a peptide within the C-terminal region of human GLI1. |
| Full Name | GLI1 antibody (pAb) |
| Synonyms | GLI1, Glioma-associated oncogene 1, zinc finger transcription factor, oncogene, hedgehog, TGF-b, cell cycle progression, neural stem cells, dbdabs, stem cell, stem cells, sample |
| Molecular weight | 120 kDa |
| GenBank accession number | NP_005260 |
| RRID | AB_2614953 |
| Purification Method | Affinity Purified |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
GLI1 (Glioma-associated oncogene 1) is a zinc finger transcription factor and oncogene. GLI1 is part of the hedgehog signaling pathway, but is also activated in response to TGF-b signaling as well. GLI1 activates transcription of a number of genes that promote cell cycle progression. GLI1 is overexpressed as a result of constitutive activation of hedgehog signaling, which plays a role in the initiation of cancer. GLI1 also promotes self-renewal of neural stem cells.