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FancD2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

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Cat No : 61057,61058 61057

Synonyms

FancD2, Fanconi anemia complementation group D2, Fanconi anemia, a recessive genetic disorder characterized by chromosomal instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair, DNA damage checkpoints, B-cell immunoglobulin isotype switching, dna damage, dna repair, western blotting, wb, immunoprecipitation, ip, antibody, antibodies, polyclonal, pab, sample

Validation Data Gallery

  • FancD2 antibody (pAb) tested by Immunoprecipitation. 10 ul of FancD2 antibody was used to immunoprecipitate FancD2 from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FancD2 antibody at a dilution of 1:500.

    FancD2 antibody (pAb) tested by Immunoprecipitation. 10 ul of FancD2 antibody was used to immunoprecipitate FancD2 from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FancD2 antibody at a dilution of 1:500.

  • Western blot of FancD2 antibody. Lane 1: HeLa whole-cell extract (40 ug). Lane 2: Nuclear extract (40 ug) of K-562 cells. Both probed with FancD2 antibody (pAb) at a 1:500 dilution.

    Western blot of FancD2 antibody. Lane 1: HeLa whole-cell extract (40 ug). Lane 2: Nuclear extract (40 ug) of K-562 cells. Both probed with FancD2 antibody (pAb) at a 1:500 dilution.

Product Information

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB: 1:500 - 1:5,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This FancD2 antibody was raised against a peptide within the internal region of human FancD2.
Full Name FancD2 antibody (pAb)
Synonyms FancD2, Fanconi anemia complementation group D2, Fanconi anemia, a recessive genetic disorder characterized by chromosomal instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair, DNA damage checkpoints, B-cell immunoglobulin isotype switching, dna damage, dna repair, western blotting, wb, immunoprecipitation, ip, antibody, antibodies, polyclonal, pab, sample
Molecular weight 150 kDa
GenBank accession numberNP_149075
RRIDAB_2793491
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

FancD2 (Fanconi anemia complementation group D2) is a protein encoded by a gene mutated in Fanconi anemia, a recessive genetic disorder characterized by chromosomal instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. FancD2 is monoubiquitinated in response to DNA damage and is required for maintenance of chromosomal stability and the repair of DNA double-strand breaks. FancD2 also likely plays a role in activating the S-phase and G2/M DNA damage checkpoints. It promotes the loading of BRCA2 and FANCD1 onto damaged chromatin and may also be involved in B-cell immunoglobulin isotype switching.