• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FTO antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Activ Motif logo

Cat No : 61431,61432 61431

Synonyms

FTO, Fat Mass and Obesity Associated, Alpha-Ketoglutarate-Dependent Dioxygenase FTO, Fat Mass And Obesity-Associated Protein, Protein Fto, DNA, RNA, damage, repair, demethylation, antibody, polyclonal, pab, western blotting, sample

Validation Data Gallery

  • FTO antibody (pAb) tested by Western blot. Detection of FTO by Western blot analysis (30 ug per lane). Lane 1: Nuclear extract of Caco-2 cells. Lane 2: Nuclear extract of HEK-293 cells. Both probed with FTO antibody (pAb) at a 1:500 dilution.

    FTO antibody (pAb) tested by Western blot. Detection of FTO by Western blot analysis (30 ug per lane). Lane 1: Nuclear extract of Caco-2 cells. Lane 2: Nuclear extract of HEK-293 cells. Both probed with FTO antibody (pAb) at a 1:500 dilution.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB: 1:500 - 1:2,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of human FTO.
Full Name FTO antibody (pAb)
Synonyms FTO, Fat Mass and Obesity Associated, Alpha-Ketoglutarate-Dependent Dioxygenase FTO, Fat Mass And Obesity-Associated Protein, Protein Fto, DNA, RNA, damage, repair, demethylation, antibody, polyclonal, pab, western blotting, sample
Molecular weight 60 kDa
GenBank accession numberNP_001073901
RRIDAB_2793633
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

FTO (fat mass and obesity associated) is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. This protein has high activity towards single-stranded RNA containing 3-methyluracil and single-stranded DNA containing 3-methylthymine. FTO has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine and has no activity towards 1-methylguanine or double-stranded DNA. FTO contributes to the regulation of the global metabolic rate, energy expenditure, energy homeostasis, regulation of body size and body fat accumulation. FTO has recently been shown to have the highest activity towards demethylation of 6-methyladeninine in single-stranded RNA suggesting that FTO is involved in processing of nuclear RNAs and may play an important role in RNA epigenetics.