FOXO3 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB

Cat No : 61593,61594

Synonyms

FOXO3, Forkhead Box O3, FOXO2, FKHRL1, Forkhead Box O3A, FOXO3A, Forkhead Box Protein O3, Forkhead In Rhabdomyosarcoma-Like 1, Forkhead Homolog (Rhabdomyosarcoma) Like 1, AF6q21, Forkhead Drosophila Homolog Of In Rhabdomyosarcoma-Like 1, FKHRL1P2, AF6q21 Protein, transcription, activator, regulation, myc, antibody, antibodies, pab, polyclonal, western blotting, wb, sample

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Validation Data Gallery

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  FOXO3 antibody (pAb) tested by Western blot. Jurkat cytosolic cell extract (20 ug) probed with FOXO3 antibody at a dilution of 1:500.

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This antibody was raised against a peptide within the N-terminal region of human FOXO3.
• Full Name: FOXO3 antibody (pAb)
• Synonyms: FOXO3, Forkhead Box O3, FOXO2, FKHRL1, Forkhead Box O3A, FOXO3A, Forkhead Box Protein O3, Forkhead In Rhabdomyosarcoma-Like 1, Forkhead Homolog (Rhabdomyosarcoma) Like 1, AF6q21, Forkhead Drosophila Homolog Of In Rhabdomyosarcoma-Like 1, FKHRL1P2, AF6q21 Protein, transcription, activator, regulation, myc, antibody, antibodies, pab, polyclonal, western blotting, wb, sample
• Molecular weight: 85 kDa
• GenBank accession number: NP_001446
• RRID: AB_2793692
• Purification Method: Affinity Purified
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

FOXO3 (forkhead box protein O3) is a transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.