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EZH2 antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human, Mouse

Applications

ChIP, ChIP-Seq, IP

Activ Motif logo

Cat No : 39002,39901,39902 39901

Synonyms

EZH2, EZH-2, EZ H2, PRC2, pAb, polyclonal, Enhancer of Zeste Homologue 2, antibody, antibodies, polycomb, Enhancer of Zeste, 39901, chip, chromatin immunoprecipitation, stem, 39639, sample

Validation Data Gallery

  • EZH2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 4 million SUDHL6 B cell lymphoma cells and 5 ul of EZH2 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 25 million sequence tags were mapped to identify EZH2 binding sites. The image on the left shows EZH2 binding at the 5’ end of the CCND2 gene. The image on the right shows EZH2 binding across the repressed HOX gene cluster.

    EZH2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with chromatin from 4 million SUDHL6 B cell lymphoma cells and 5 ul of EZH2 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 25 million sequence tags were mapped to identify EZH2 binding sites. The image on the left shows EZH2 binding at the 5’ end of the CCND2 gene. The image on the right shows EZH2 binding across the repressed HOX gene cluster.

Product Information

Tested Applications ChIP, ChIP-Seq, IP

Applications Validated by Active Motif: ChIP-Seq: 5 ul each This antibody has not been validated in WB, but a WB-validated antibody is available for this target: 39933. For EZH2, we also offer AbFlex EZH2 Recombinant Antibody (rAb). For details, see Catalog No. 91159.
Tested Reactivity Human, Mouse
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
Immunogen This EZH2 antibody was raised against a recombinant fusion protein corresponding to amino acids 1-370 of mouse EZH2.
Full Name EZH2 antibody (pAb)
Synonyms EZH2, EZH-2, EZ H2, PRC2, pAb, polyclonal, Enhancer of Zeste Homologue 2, antibody, antibodies, polycomb, Enhancer of Zeste, 39901, chip, chromatin immunoprecipitation, stem, 39639, sample
Molecular weight 96 kDa
GenBank accession numberNP_031997
RRIDAB_2614956
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 61641) of this antibody that was purified by Protein A Chromatography is also available.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

EZH2 – (Enhancer of Zeste homolog 2) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.