EZH1 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Mouse
Applications
WB
Cat No : 61583,61584 61583
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB*: 1:1,000 - 1:2,500 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. |
| Tested Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This antibody was raised against a peptide within the N-terminal region of human EZH1. |
| Full Name | EZH1 antibody (pAb) |
| Synonyms | EZH1, KMT6B, Enhancer Of Zeste Homolog 1 (Drosophila), ENX-2, Histone-Lysine N-Methyltransferase EZH1, EC 2.1.1.43, KIAA0388, Enhancer Of Zeste (Drosophila) Homolog 1, Enhancer Of Zeste Homolog 1, histone, histone h3, h3, h3k27me1, h3k27me2, h3k27me3, lysine 27, lys-27, prc2, eed, polycomb, methyltransferase, polyclonal, pab, antibody, antibodies, western blotting, wb, sample |
| Molecular weight | 100 kDa |
| GenBank accession number | NP_001982 |
| RRID | AB_2793687 |
| Purification Method | Affinity Purified |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
EZH1 (Enhancer Of Zeste Homolog 1 ) is a Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.