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Drosha antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

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Cat No : 39783,39784 39783

Synonyms

RNASE3L, Drosha, RNAi, miRNA, pri-miRNA, siRNA, rna interference, dicer, rnase, rna processing, microprocessor, RISC, RNAse III, western blotting, wb, immunprecipitation, ip, polyclonal, pab, antibody, sample

Validation Data Gallery

  • Drosha antibody (pAb) tested by Immunoprecipitation. 10 ul of Drosha antibody was used to immunoprecipitate Drosha from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Drosha antibody at a dilution of 1:500.

    Drosha antibody (pAb) tested by Immunoprecipitation. 10 ul of Drosha antibody was used to immunoprecipitate Drosha from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Drosha antibody at a dilution of 1:500.

  • Drosha antibody (pAb) tested by Western blot. HeLa cell nuclear extract (40 ug) probed with Drosha antibody at a dilution of 1:500.

    Drosha antibody (pAb) tested by Western blot. HeLa cell nuclear extract (40 ug) probed with Drosha antibody at a dilution of 1:500.

Product Information

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB*: 1:500 - 1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This Drosha antibody was raised against a peptide within the C-terminal region of human Drosha.
Full Name Drosha antibody (pAb)
Synonyms RNASE3L, Drosha, RNAi, miRNA, pri-miRNA, siRNA, rna interference, dicer, rnase, rna processing, microprocessor, RISC, RNAse III, western blotting, wb, immunprecipitation, ip, polyclonal, pab, antibody, sample
Molecular weight 160 kDa
GenBank accession numberNP_037367
RRIDAB_2793342
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Drosha is an RNAse III enzyme that is involved in the process of generating microRNA (miRNA). miRNA interacts with the RNA-induced silencing complex (RISC) to cleave mRNA and thus downregulate gene expression. Drosha is a double stranded RNAse and part of the Microprocessor complex. Other RNAse III enzymes include Dicer and Argonaute. Drosha initiates processing of the miRNA precursor (pri-miRNA) in the nucleus, which is then exported to the cytoplasm and further cleaved by Dicer into a mature miRNA.