Dicer antibody (mAb) (Clone N167/7)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
WB
CloneNo.
N167/7
Cat No : 39817,39818 39817
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 1 - 2 ug/ml dilution This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91241. |
| Tested Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This Dicer antibody was raised against a recombinant protein corresponding to amino acids 1638-1899 of mouse Dicer. |
| Full Name | Dicer antibody (mAb) (Clone N167/7) |
| Synonyms | dicer, rnase, rnai, silencing, sirna, mirna, pri-mirna, risc, rna interference, PTSG, RISC, sample |
| Molecular weight | 245 kDa |
| GenBank accession number | NP_683750 |
| RRID | AB_2793353 |
| Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Dicer is a double-stranded RNase and part of the micro-RNA (miRNA) loading complex (miRLC), which is composed of Dicer, Ago2 and TARBP2. Dicer is required for formation of the RNA induced silencing complex (RISC). Dicer and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto Ago2. Dicer also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the destruction of complementary RNAs.