DNMT1 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF, IP, WB
Cat No : 39905,39906 39905
Synonyms
Validation Data Gallery
Product Information
Tested Applications |
IF, IP, WB
Applications Validated by Active Motif: IP: 10 ug per IP WB*: 0.5 - 2 ug/ml dilution IF: 1:500 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. For DNMT1, we also offer AbFlex DNMT1 Recombinant Antibody (rAb). For details, see Catalog No. 91175. |
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | This DNMT1 antibody was raised against a recombinant protein corresponding to amino acids 1-110 of human DNMT1. |
Full Name | DNMT1 antibody (pAb) |
Synonyms | DNMT1, DNMT-1, DNMT 1, DNA Methyltransferase 1, antibody, antibodies, DNA methylation, stem cell, stem cells, western blotting, wb, immunoprecipitation, ip, polyclonal, pab, sample |
Molecular weight | 185 kDa |
GenBank accession number | NP_001370 |
RRID | AB_2793389 |
Purification Method | Protein A Chromatography |
Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.