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DNMT1 antibody (mAb) (Clone 60B1220.1)

Host / Isotype

Mouse / IgG

Reactivity

Human, Mouse

Applications

ChIP, IHC, IP, WB

CloneNo.

60B1220.1

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Cat No : 39204 39204

Synonyms

DNMT1, DNMT-1, DNMT 1, mAb, monoclonal, DNA Methyltransferase 1, antibody, antibodies, chip, chromatin immunoprecipitation

Validation Data Gallery

  • DNMT1 antibody (mAb) tested by Western blot. Detection of DNMT1 by Western blot. The analysis was performed using mouse ES whole-cell extract and DNMT1 antibody (mAb) at a 2 ug/ml dilution.

    DNMT1 antibody (mAb) tested by Western blot. Detection of DNMT1 by Western blot. The analysis was performed using mouse ES whole-cell extract and DNMT1 antibody (mAb) at a 2 ug/ml dilution.

Product Information

Tested Applications ChIP, IHC, IP, WB

Applications Validated by Active Motif: WB: 2 - 4 ug/ml dilution For DNMT1, we also offer AbFlex DNMT1 Recombinant Antibody (rAb). For details, see Catalog No. 91175.
Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG
Class Monoclonal
Type Antibody
Immunogen This DNMT1 antibody was raised against a peptide corresponding to amino acid residues 637-651 of human DNMT1.
Full Name DNMT1 antibody (mAb) (Clone 60B1220.1)
Synonyms DNMT1, DNMT-1, DNMT 1, mAb, monoclonal, DNA Methyltransferase 1, antibody, antibodies, chip, chromatin immunoprecipitation
Molecular weight 190 kDa
GenBank accession numberNP_001370
RRIDAB_2614950
Purification Method Affinity Purified
Buffer PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.