DNMT1 antibody (mAb) (Clone 2C1)
Host / Isotype
Rat / IgG2b
Reactivity
Human
Applications
ChIP, IP, WB
CloneNo.
2C1
Cat No : 61467,61468 61467
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
ChIP, IP, WB
This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91175. |
| Tested Reactivity | Human |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This antibody was raised against a full-length recombinant protein corresponding to human DNMT1. |
| Full Name | DNMT1 antibody (mAb) (Clone 2C1) |
| Synonyms | DNMT1, DNMT 1, DNA methyltransferase 1, DNA (Cytosine-5-)-Methyltransferase 1, DNA MTase HsaI, MCMT, CXXC9, HSN1E, DNMT, DNA (Cytosine-5)-Methyltransferase 1, AIM, M.HsaI, CXXC-Type Zinc Finger Protein 9, Dnmt1, DNA Methyltransferase HsaI, M.HsaI, DNA methylation, antibody, antibodies, monoclonal, chromatin immunoprecipitation, chip, immunoprecipitation, ip, western blotting, wb, stem cell, stem cells, sample |
| Molecular weight | 190 kDa |
| GenBank accession number | NP_001124295 |
| RRID | AB_2614954 |
| Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.