DMAP1 antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IP, WB
Cat No : 61675,61676 61675
Synonyms
Validation Data Gallery
Product Information
Tested Applications |
IP, WB
Applications Validated by Active Motif: IP: 10 ul per IP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. |
Tested Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | This antibody was raised against a peptide within the C-terminal region of human DMAP1. |
Full Name | DMAP1 antibody (pAb) |
Synonyms | DMAP1, DNA Methyltransferase 1 Associated Protein 1, MEAF2, DNMAP1, SWC4, DNMT1-Associated Protein 1, DNA Methyltransferase 1-Associated Protein 1, DNMTAP1, DNMT1 Associated Protein 1, EAF2, KIAA1425, HDAC2, transcription, transcriptional repression, transcriptional activation, DAXX, GR, NuA4 histone acetyltransferase complex, antibody, antibodies, polyclonal, pab, western blotting, wb, immunoprecipitation, ip, sample |
Molecular weight | 60 kDa |
GenBank accession number | NP_061973 |
RRID | AB_2793730 |
Purification Method | Affinity Purified |
Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
DMAP1 (DNA Methyltransferase 1 Associated Protein 1) is involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.