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DHX9 antibody (mAb) (Clone 8E3)

Host / Isotype

Rat / IgG1

Reactivity

Human, Mouse

Applications

ICC, IF, WB

CloneNo.

8E3

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Cat No : 61705,61706 61705

Synonyms

DHX9, DEAH (Asp-Glu-Ala-His) Box Helicase 9, NDHII, DDX9, DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 9 (RNA Helicase A, Nuclear DNA Helicase II; Leukophysin), LKP, ATP-Dependent RNA Helicase A, DEAH (Asp-Glu-Ala-His) Box Polypeptide 9, DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 9, NDH II, leukophysin, RNA Helicase A, EC 3.6.4.13, Nuclear DNA Helicase II, Leukophysin, NDH2, EC 3.6.1, RHA, EC 3.6.1.15, DEAH Box Protein 9, transcriptional activator, monoclonal, mab, antibody, antibodies, immunofuorescence, if, immunocytochemistry, icc, western blotting, wb, sample

Validation Data Gallery

  • DHX9 antibody (mAb) (Clone 8E3) tested by Immunofluorescence. Left: Formaldehyde fixed L929 cells stained with DHX9 antibody (mAb). Right: Hoechst.

    DHX9 antibody (mAb) (Clone 8E3) tested by Immunofluorescence. Left: Formaldehyde fixed L929 cells stained with DHX9 antibody (mAb). Right: Hoechst.

  • DHX9 antibody (mAb) (Clone 8E3) tested by Western blot. DHX9 antibody detection by Western blot. The analysis was performed using 30 ug of either NIH-3T3 nuclear extract (lane 1), NIH-3T3 cytoplasmic extract (lane 2), or HeLa nuclear extract (lane 3) and DHX9 antibody (mAb) at a 2 ug/ml dilution.

    DHX9 antibody (mAb) (Clone 8E3) tested by Western blot. DHX9 antibody detection by Western blot. The analysis was performed using 30 ug of either NIH-3T3 nuclear extract (lane 1), NIH-3T3 cytoplasmic extract (lane 2), or HeLa nuclear extract (lane 3) and DHX9 antibody (mAb) at a 2 ug/ml dilution.

Product Information

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 2 - 10 ug/ml dilution WB: 0.5 - 2 ug/ml dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
Tested Reactivity Human, Mouse
Host / Isotype Rat / IgG1
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the N-terminal region of mouse DHX9.
Full Name DHX9 antibody (mAb) (Clone 8E3)
Synonyms DHX9, DEAH (Asp-Glu-Ala-His) Box Helicase 9, NDHII, DDX9, DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 9 (RNA Helicase A, Nuclear DNA Helicase II; Leukophysin), LKP, ATP-Dependent RNA Helicase A, DEAH (Asp-Glu-Ala-His) Box Polypeptide 9, DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 9, NDH II, leukophysin, RNA Helicase A, EC 3.6.4.13, Nuclear DNA Helicase II, Leukophysin, NDH2, EC 3.6.1, RHA, EC 3.6.1.15, DEAH Box Protein 9, transcriptional activator, monoclonal, mab, antibody, antibodies, immunofuorescence, if, immunocytochemistry, icc, western blotting, wb, sample
Molecular weight 150 kDa
GenBank accession numberNP_031868
RRIDAB_2793743
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

DHX9 (DEAH (Asp-Glu-Ala-His) Box Helicase 9) unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Positively regulates HIV-1 LTR-directed gene expression.